Published August 25, 2021 | Version v1
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Data from: Molecular phylogeny and taxonomic revision of the genus Diastatea (Campanulaceae: Lobelioideae)

  • 1. Northern Arizona University

Description

Diastatea (Campanulaceae-Lobelioideae) is a genus of small, herbaceous annuals found mostly in Mexico. Differences in the number of recognized species in the historical treatments, and the lack of a phylogeny, suggested that a revision of the genus was necessary. Species boundaries and species level relationships were tested based on a sampling of nearly 50 individuals. Sequences of the chloroplast spacer regions, atpB-rbcL and ndhF-rpl32, as well as the ITS, were generated and then analyzed using maximum likelihood and Bayesian inference methods. The analyses of the combined dataset produced congruent topologies. Two strongly supported clades of Diastatea are newly recognized as subgenera in the taxonomic treatment. Diastatea Scheidw. subg. Diastatea and Diastatea subg. Angustifolia E.P.Johnson and T.J.Ayers can be distinguished morphologically by leaf shape and pubescence, hypanthium shape, and the presence or absence of a gibbous projection on the ventral side of the corolla. A morphological treatment of the genus corroborated by the molecular analysis revealed one new species, Diastatea aptera E.P.Johnson & T.J.Ayers, one new subspecies, Diastatea tenera subsp. durangensis E.P.Johnson & T.J.Ayers, and elevated an existing subspecies, Diastatea ciliata (McVaugh) E.P.Johnson & T.J.Ayers, to specific rank.

Notes

ReadMe file can be found with data download.

Figure Captions for Supplemental Figures and Table:

Fig. S1.  Maximum likelihood tree for the concatenated dataset with outgroup nodes expanded to show specimen information. Numbers above branches represent bootstrap values followed by posterior probabilities. Posterior probabilities below 0.95 are shown as dashes. 

Fig. S2. Maximum likelihood tree for the ITS dataset. Numbers above branches represent bootstrap values. 

Fig. S3. Maximum likelihood tree for the concatenated chloroplast dataset which includes ndhF-rpl32 and atpB-rbcL. Numbers above branches represent bootstrap values. 

Fig. S4. Scanning electron micrograph images of seed coat surface in Diastatea. A. D. aptera (Ayers et al. 1767).B. D. tenera subsp. durangensis (Jiménez 186). C. D. ciliata (Reveal et al. 4183). D. D. micrantha (Ayers et al. 1766)with striations obvious due to incomplete filling of seed.

Fig. S5. Photos of chromosomes obtained from root tips. A. Diastatea aptera, 2n = 14. B. D. virgata, 2n = 14. C. D. micrantha, 2n =14.

Table S1. Number of specimens out of total available where data were obtained. 

Alignment of Total Concatenated Dataset: Sequences obtained from atpB-rbcL and ndhF-rpl32 regions of the chloroplast, and the nrITS region, were concatenated into one alignment for use in the phylogenetic analysis. 

Alignment of ITS Dataset: Sequences obtained from the nrITS region were aligned to perform a phylogenetic analysis. 

Alignment of Concatenated Chloroplast Dataset: Sequences obtained from atpB-rbcL and ndhF-rpl32 regions of the chloroplast were concatenated and aligned to perform a phylogenetic analysis. 

Files

Fig_S1_JohnsonAyers_2021_DiastateaRevision_CombinedMLTreeOutgroupsExpanded.pdf

Additional details

Related works

Is derived from
10.5061/dryad.rr4xgxd89 (DOI)