A new type of mouse gaze shift is led by directed saccades
Authors/Creators
- 1. University of California, San Francisco
Description
Animals investigate their environments by directing their gaze towards salient stimuli. In the prevailing view, mouse gaze shifts are led by head rotations that trigger compensatory, brainstem-mediated eye movements, including saccades to reset the eyes. These "recentering" saccades are attributed to head movement-related vestibular and optokinetic cues. However, microstimulating mouse superior colliculus (SC) elicits directed head and eye movements that resemble SC-dependent sensory-guided gaze shifts made by other species, raising the possibility mice generate additional types of gaze shifts. We investigated this possibility by tracking eye and attempted head movements in a head-fixed preparation that eliminates head movement-related sensory cues. We found tactile stimuli evoke gaze shifts involving directed saccades that precede attempted head rotations. Optogenetic perturbations revealed SC drives touch-evoked gaze shifts. Thus, mice make sensory-guided, SC-dependent gaze shifts led by directed saccades. Our findings uncover diversity in mouse gaze shifts and provide a foundation for studying head-eye coupling.
Notes
DeepLabCut reports prediction confidence using likelihood values. Rather than analyze faulty data points, frames containing any points with DeepLabCut-calculated likelihood of P < 0.99 have been set to NaNs.
Sessions from the same mouse under the same stimulation protocol have been combined into single time series.
For our paper, mice were excluded from analysis for a given stimulus type (e.g., whisker airpuff) if the probability of a saccade during the response window (within 150 ms of stimulus) across all sessions was below 5%. We have not excluded mice or sessions from this dataset.
Funding provided by: National Institute of Mental Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000025
Award Number: DP2 MH119426
Funding provided by: National Institute of Neurological Disorders and Stroke
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000065
Award Number: R01 NS109060
Funding provided by: Simons Foundation Autism Research Initiative
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100014370
Award Number: 574347
Funding provided by: Klingenstein-Simons Fellowship Award in Neuroscience*
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Funding provided by: Whitehall Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100001391
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Funding provided by: E. Matilda Ziegler Foundation for the Blind
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100010310
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Funding provided by: Brain and Behavior Research Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000874
Award Number: 25337
Funding provided by: Brain and Behavior Research Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000874
Award Number: 27320
Funding provided by: Sandler Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100007100
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Mice_Make_Targeted_Saccades.zip
Additional details
Related works
- Is cited by
- 10.7554/eLife.73081 (DOI)