RNA m6A methylation in unilateral ureteral ligations model

Chronic kidney disease (CKD) is a high incidence disease. Renal fibrosis is the ultimate pathological pathway for chronic kidney diseases leading to end-stage renal failure. There is currently no effective treatment to prevent the renal fibrosis. Renal EMT is a key mechanism of renal fibrosis. Recent evidence suggests m⁶A modification in RNA can regulate EMT in tumor cells. However, a role of m⁶A RNA modification in renal fibrosis has not been reported. Therefore, we investigate whether m⁶A RNA methylation plays an important role in the epithelial-to-mesenchymal transition (EMT) process of renal fibrosis. Unilateral ureteral ligations were performed in C57BL/6 mice to establish a mouse model of renal fibrosis and TGF-b intervention was used in HK2 cells to establish a cellular model of renal fibrosis. The effects of m⁶A and the role of Mettl14 on kidney disease and HK2 cells were studied.