Published December 21, 2020 | Version v1

Fleas are parasitic scorpionflies

Description

Tihelka, Erik, Giacomelli, Mattia, Huang, Di-Ying, Pisani, Davide, Donoghue, Philip C. J., Cai, Chen-Yang (2020): Fleas are parasitic scorpionflies. Palaeoentomology 3 (6): 641-653, DOI: 10.11646/palaeoentomology.3.6.16

Files

Restricted

The record is publicly accessible, but files are restricted. Log in to check if you have access.

Linked records

Additional details

References

  • Bajdek, P., Qvarnstrom, M., Owocki, K., Sulej, T., Sennikov, A.G., Golubev, V.K. & Niedzwiedzki, G. (2016) Microbiota and food residues including possible evidence of pre-mammalian hair in Upper Permian coprolites from Russia. Lethaia, 49, 455-477. https://doi.org/10.1111/let.12156
  • Beutel, R.G. & Baum, E. (2008) A longstanding entomological problem finally solved? Head morphology of Nannochorista (Mecoptera, Insecta) and possible phylogenetic implications. Journal of Zoological Systematics and Evolutionary Research, 46, 346-367. https://doi.org/10.1111/j.1439-0469.2008.00473.x
  • Beutel, R.G. & Friedrich, F. (2019) Phylogeny. In: Beutel, R.G. & Friedrich, F. (Eds). Nannomecoptera and Neomecoptera. Handbook of Zoology: Arthropoda: Insecta. De Gruyter, Berlin, Boston, pp. 159-162. https://doi.org/10.1515/9783110272543-004
  • Beutel, R.G., Friedrich, F., Hornschemeyer, T., Pohl, H., Hunefeld, F., Beckmann, F., Meier, R., Misof, B., Whiting, M.F. & Vilhelmsen, L. (2011) Morphological and molecular evidence converge upon a robust phylogeny of the megadiverse Holometabola. Cladistics, 27, 341-355. https://doi.org/10.1111/j.1096-0031.2010.00338.x
  • Beutel, R.G., Friedrich, F., Yang, X.K. & Ge, S.Q. (2013) Insect morphology and phylogeny: A textbook for students of entomology. Walter de Gruyter, 532 pp. https://doi.org/10.1515/9783110264043
  • Beutel, R.G. & Pohl, H. (2006) Endopterygote systematics - where do we stand and what is the goal (Hexapoda, Arthropoda)? Systematic Entomology, 31, 202-219. https://doi.org/10.1111/j.1365-3113.2006.00341.x
  • Beutel, R.G., Yavorskaya, M.I., Mashimo, Y., Fukui, M. & Meusemann, K. (2017) The phylogeny of Hexapoda (Arthropoda) and the evolution of megadiversity. Proceedings of Arthropodan Embryological Society of Japan, 51, 1-15.
  • Bilinski, S.M. & Buning, J. (1998) Structure of ovaries and oogenesis in the snow scorpionfly Boreus hyemalis (Linne) (Mecoptera: Boreidae). International Journal of Insect Morphology and Embryology, 27, 333-340. https://doi.org/10.1016/S0020-7322(98)00026-9
  • Borner, C. (1904) Zur Systematik der Hexapoda. Zoologischer Anzeiger, 27, 511- 533.
  • Boudreaux, H.B. (1979) Arthropod phylogeny with special reference to insects. Wiley-Interscience, New York, 320 pp.
  • Byers, G.W. (1996) More on the origin of Siphonaptera. Journal of the Kansas Entomological Society, 69, 274-277.
  • Byers, G.W. (2009) Mecoptera: scorpionflies, hangingflies. In: V. H. Resh and R. T. Carde (Eds). Encyclopedia of insects (Second edition). Academic Press, San Diego, pp. 611-614. https://doi.org/10.1016/B978-0-12-374144-8.00170-3
  • Chalwatzis, N., Hauf, J., Van De Peer, Y., Kinzelbach, R. & Zimmermann, F.K. (1996) 18S ribosomal RNA genes of insects: primary structure of the genes and molecular phylogeny of the Holometabola. Annals of the Entomological Society of America, 89, 788-803. https://doi.org/10.1093/aesa/89.6.788
  • Criscuolo, A. & Gribaldo, S. (2010) BMGE (Block Mapping and Gathering with Entropy): a new software for selection of phylogenetic informative regions from multiple sequence alignments. BMC Evolutionary Biology, 10, 210. https://doi.org/10.1186/1471-2148-10-210
  • Dallai, R., Lupetti, P., Afzelius, B.A. & Frati, F. (2003) Sperm structure of Mecoptera and Siphonaptera (Insecta) and the phylogenetic position of Boreus hyemalis. Zoomorphology, 122, 211-220. https://doi.org/10.1007/s00435-003-0087-y
  • Feuda, R., Dohrmann, M., Pett, W., Philippe, H., Rota-Stabelli, O., Lartillot, N., Worheide, G. & Pisani, D. (2017) Improved modeling of compositional heterogeneity supports sponges as sister to all other animals. Current Biology, 27, 3864- 3870.e4. https://doi.org/10.1016/j.cub.2017.11.008
  • Fraulob, M., Wipfler, B., Hunefeld, F., Pohl, H. & Beutel, R.G. (2012) The larval abdomen of the enigmatic Nannochoristidae (Mecoptera, Insecta). Arthropod Structure & Development, 41, 187-198. https://doi.org/10.1016/j.asd.2011.11.001
  • Hennig, W. (1969) Die Stammesgeschichte der Insekten. 1st ed. Waldemar Kramer, Frankfurt, 436 pp.
  • Hinton, H.E. (1958) The phylogeny of the panorpoid orders. Annual Review of Entomology, 3, 181-206. https://doi.org/10.1146/annurev.en.03.010158.001145
  • Hinton, H.E. (1981) Biology of insect eggs. Vol. II. Pergamon Press, Oxford, 778 pp.
  • Huang, D.Y., Engel, M.S., Cai, C.Y. & Nel, A. (2013) Mesozoic giant fleas from northeastern China (Siphonaptera): Taxonomy and implications for palaeodiversity. Chinese Science Bulletin, 58, 1682-1690. https://doi.org/10.1007/s11434-013-5769-3
  • Huang, D.Y., Engel, M.S., Cai, C.Y., Wu, H. & Nel, A. (2012) Diverse transitional giant fleas from the Mesozoic era of China. Nature, 483, 201-204. https://doi.org/10.1038/nature10839
  • Inagaki, Y. & Roger, A.J. (2006) Phylogenetic estimation under codon models can be biased by codon usage heterogeneity. Molecular Phylogenetics and Evolution, 40, 428-434. https://doi.org/10.1016/j.ympev.2006.03.020
  • Inward, D., Beccaloni, G. & Eggleton, P. (2007) Death of an order: a comprehensive molecular phylogenetic study confirms that termites are eusocial cockroaches. Biology Letters, 3, 331-335. https://doi.org/10.1098/rsbl.2007.0102
  • Ishiwata, K., Sasaki, G., Ogawa, J., Miyata, T. & Su, Z.H. (2011) Phylogenetic relationships among insect orders based on three nuclear protein-coding gene sequences. Molecular Phylogenetics and Evolution, 58, 169-180. https://doi.org/10.1016/j.ympev.2010.11.001
  • Johnson, K.P., Dietrich, C.H., Friedrich, F., Beutel, R.G., Wipfler, B., Peters, R.S., Allen, J.M., Petersen, M., Donath, A., Walden, K.K.O., Kozlov, A.M., Podsiadlowski, L., Mayer, C., Meusemann, K., Vasilikopoulos, A., Waterhouse, R.M., Cameron, S.L., Weirauch, C., Swanson, D.R., Percy, D.M., Hardy, N.B., Terry, I., Liu, S., Zhou, X., Misof, B., Robertson, H.M. & Yoshizawa, K. (2018) Phylogenomics and the evolution of hemipteroid insects. Proceedings of the National Academy of Sciences, 115, 12775-12780. https://doi.org/10.1073/pnas.1815820115
  • Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587-589. https://doi.org/10.1038/nmeth.4285
  • Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30, 772- 780. https://doi.org/10.1093/molbev/mst010
  • Kjer, K.M. (2004) Aligned 18S and insect phylogeny. Systematic Biology, 53, 506-514. https://doi.org/10.1080/10635150490445922
  • Kjer, K.M., Carle, F.L., Litman, J. & Ware, J. (2006) A molecular phylogeny of Insecta. Arthropod Systematics and Phylogeny, 64, 35-44.
  • Kjer, K.M., Simon, C., Yavorskaya, M. & Beutel, R.G. (2016) Progress, pitfalls and parallel universes: a history of insect phylogenetics. Journal of The Royal Society Interface, 13, 20160363. https://doi.org/10.1098/rsif.2016.0363
  • Kristensen, N.P. (1975) The phylogeny of hexapod "orders". A critical review of recent accounts. Journal of Zoological Systematics and Evolutionary Research, 13, 1-44. https://doi.org/10.1111/j.1439-0469.1975.tb00226.x
  • Kristensen, N.P. (1981) Phylogeny of insect orders. Annual Review of Entomology, 26, 135-157. https://doi.org/10.1146/annurev.en.26.010181.001031
  • Kristensen, N.P. (1991) Phylogeny of extant hexapods. In: CSIRO Division of Entomology. The insects of Australia: a textbook for students and research workers. 2nd ed. Vol. 1. Melbourne University Press, Carlton, pp. 125-140.
  • Kristensen, N.P. (1999) Phylogeny of endopterygote insects, the most successful lineage of living organisms. European Journal of Entomology, 96, 237-253.
  • Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35, 1547-1549. https://doi.org/10.1093/molbev/msy096
  • Lartillot, N., Brinkmann, H. & Philippe, H. (2007) Suppression of long-branch attraction artefacts in the animal phylogeny using a site-heterogeneous model. BMC Evolutionary Biology, 7, S4. https://doi.org/10.1186/1471-2148-7-S1-S4
  • Lartillot, N., Lepage, T. & Blanquart, S. (2009) PhyloBayes 3: a Bayesian software package for phylogenetic reconstruction and molecular dating. Bioinformatics, 25, 2286-2288. https://doi.org/10.1093/bioinformatics/btp368
  • Lewis, R.E. (1998) Resume of the Siphonaptera (Insecta) of the world. Journal of Medical Entomology, 35, 377-389. https://doi.org/10.1093/jmedent/35.4.377 Marletaz, F., Peijnenburg, K.T.C.A., Goto, T., Satoh, N. & Rokhsar, D.S. (2019) A new spiralian phylogeny places the enigmatic arrow worms among gnathiferans. Current Biology, 29, 312-318. https://doi.org/10.1016/j.cub.2018.11.042
  • Lewis, R.E. (1998) Resume of the Siphonaptera (Insecta) of the world. Journal of Medical Entomology, 35, 377-389. https://doi.org/10.1093/jmedent/35.4.377 Marletaz, F., Peijnenburg, K.T.C.A., Goto, T., Satoh, N. & Rokhsar, D.S. (2019) A new spiralian phylogeny places the enigmatic arrow worms among gnathiferans. Current Biology, 29, 312-318.
  • McKenna, D.D. & Farrell, B.D. (2010) 9-genes reinforce the phylogeny of Holometabola and yield alternate views on the phylogenetic placement of Strepsiptera. PLoS ONE, 5, e11887. https://doi.org/10.1371/journal.pone.0011887
  • Medvedev, S.G. (2017) Adaptations of fleas (Siphonaptera) to parasitism. Entomological Review, 97, 1023-1030. https://doi.org/10.1134/S0013873817080012
  • Michelsen, V. (1997) A revised interpretation of the mouthparts in adult fleas (Insecta, Siphonaptera). Zoologischer Anzeiger, 235, 217-223.
  • Mickoleit, G. (2008) Die Sperma-Auspressvorrichtung der Nannochoristidae (Insecta: Mecoptera. Entomologia Generalis, 193-226. https://doi.org/10.1127/entom.gen/31/2008/193
  • Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 Gateway Computing Environments Workshop (GCE), pp. 1-8. https://doi.org/10.1109/GCE.2010.5676129
  • Misof, B., Liu, S., Meusemann, K., Peters, R.S., Donath, A., Mayer, C., Frandsen, P.B., Ware, J., Flouri, T., Beutel, R.G., Niehuis, O., Petersen, M., Izquierdo-Carrasco, F., Wappler, T., Rust, J., Aberer, A.J., Aspock, U., Aspock, H., Bartel, D., Blanke, A., Berger, S., Bohm, A., Buckley, T.R., Calcott, B., Chen, J., Friedrich, F., Fukui, M., Fujita, M., Greve, C., Grobe, P., Gu, S., Huang, Y., Jermiin, L.S., Kawahara, A.Y., Krogmann, L., Kubiak, M., Lanfear, R., Letsch, H., Li, Y., Li, Z., Li, J., Lu, H., Machida, R., Mashimo, Y., Kapli, P., McKenna, D.D., Meng, G., Nakagaki, Y., Navarrete-Heredia, J.L., Ott, M., Ou, Y., Pass, G., Podsiadlowski, L., Pohl, H., Reumont, B.M. von, Schutte, K., Sekiya, K., Shimizu, S., Slipinski, A., Stamatakis, A., Song, W., Su, X., Szucsich, N.U., Tan, M., Tan, X., Tang, M., Tang, J., Timelthaler, G., Tomizuka, S., Trautwein, M., Tong, X., Uchifune, T., Walzl, M.G., Wiegmann, B.M., Wilbrandt, J., Wipfler, B., Wong, T.K.F., Wu, Q., Wu, G., Xie, Y., Yang, S., Yang, Q., Yeates, D.K., Yoshizawa, K., Zhang, Q., Zhang, R., Zhang, W., Zhang, Y., Zhao, J., Zhou, C., Zhou, L., Ziesmann, T., Zou, S., Li, Y., Xu, X., Zhang, Y., Yang, H., Wang, J., Wang, J., Kjer, K.M. & Zhou, X. (2014) Phylogenomics resolves the timing and pattern of insect evolution. Science, 346, 763-767. https://doi.org/10.1126/science.1257570
  • Nguyen, L.-T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32, 268-274. https://doi.org/10.1093/molbev/msu300
  • Niehuis, O., Hartig, G., Grath, S., Pohl, H., Lehmann, J., Tafer, H., Donath, A., Krauss, V., Eisenhardt, C., Hertel, J., Petersen, M., Mayer, C., Meusemann, K., Peters, R.S., Stadler, P.F., Beutel, R.G., Bornberg-Bauer, E., McKenna, D.D. & Misof, B. (2012) Genomic and morphological evidence converge to resolve the enigma of Strepsiptera. Current Biology, 22, 1309-1313. https://doi.org/10.1016/j.cub.2012.05.018
  • Palmer, C. (2010) Diversity of feeding strategies in adult Mecoptera. Terrestrial Arthropod Reviews, 3, 111-128. https://doi.org/10.1163/187498310X519716
  • Peters, R.S., Meusemann, K., Petersen, M., Mayer, C., Wilbrandt, J., Ziesmann, T., Donath, A., Kjer, K.M., Aspock, U., Aspock, H., Aberer,A., Stamatakis,A., Friedrich, F., Hunefeld, F., Niehuis, O., Beutel, R.G. & Misof, B. (2014) The evolutionary history of holometabolous insects inferred from transcriptome-based phylogeny and comprehensive morphological data. BMC Evolutionary Biology, 14, 52. https://doi.org/10.1186/1471-2148-14-52
  • Philippe, H., Brinkmann, H., Lavrov, D.V., Littlewood, D.T.J., Manuel, M., Worheide, G. & Baurain, D. (2011) Resolving difficult phylogenetic questions: why more sequences are not enough. PLoS Biology, 9, e1000602. https://doi.org/10.1371/journal.pbio.1000602
  • Pinto, I.D. & de Ornellas, L.P. (1978) New fossil insects from the White Band Formation (Permian), South Africa. Pesquisas em Geociencias, 10, 96-104. https://doi.org/10.22456/1807-9806.21778
  • Pisani, D., Pett, W., Dohrmann, M., Feuda, R., Rota-Stabelli, O., Philippe, H., Lartillot, N. & Worheide, G. (2015) Genomic data do not support comb jellies as the sister group to all other animals. Proceedings of the National Academy of Sciences, 112, 15402-15407. https://doi.org/10.1073/pnas.1518127112
  • Richards, A.G. (1965) The proventriculus of adult Mecoptera and Siphonaptera. Entomological News, 76, 253-256.
  • Richards, P.A. & Richards, A.G. (1969) Acanthae: a new type of cuticular process in the proventriculus of Mecoptera and Siphonaptera. Zoologische Jahrbucher, Abteilung fur Anatomie und Ontogenie der Tiere, 86, 158-176.
  • Rohdendorf, B.B. (1957) Basic trends in the historical development oftheDiptera.Tezisydokladov2.soveshchaniyaVesesoyuznogo Entomologicheskogo Obshchestva, 1, 115-119.
  • Ross, H. (1965) A textbook of entomology. 3rd ed. John Wiley and Sons, London, 539 pp.
  • Rota-Stabelli, O., Lartillot, N., Philippe, H. & Pisani, D. (2013) Serine codon-usage bias in deep phylogenomics: Pancrustacean relationships as a case study. Systematic Biology, 62, 121-133. https://doi.org/10.1093/sysbio/sys077
  • Rothschild, M.L., Schlein, J., Parker, K., Neville, C., Sternberg, S. & Pringle, J.W.S. (1975) The jumping mechanism of Xenopsylla cheopis III. Execution of the jump and activity. Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 271, 499-515. https://doi.org/10.1098/rstb.1975.0064
  • Schrempf, D., Lartillot, N. & Szollosi, G. (2020) Scalable empirical mixture models that account for across-site compositional heterogeneity. Molecular Biology and Evolution, msaa145. https://doi.org/10.1093/molbev/msaa145
  • Schwentner, M., Combosch, D.J., Nelson, J.P. & Giribet, G. (2017) A phylogenomic solution to the origin of insects by resolving crustacean-hexapod relationships. Current Biology, 27, 1818-1824. https://doi.org/10.1016/j.cub.2017.05.040
  • Sharov, A.G. (1966) Basic arthropodan stock, with special refer- ence to insects. 1st ed. Pergamon Press, Oxford, 271 pp.
  • Simiczyjew, B. (2002) Structure of the ovary in Nannochorista neotropica Navas (Insecta: Mecoptera: Nannochoristidae) with remarks on mecopteran phylogeny. Acta Zoologica, 83, 61-66. https://doi.org/10.1046/j.1463-6395.2002.00099.x
  • Simion, P., Philippe, H., Baurain, D., Jager, M., Richter, D.J., Di Franco, A., Roure, B., Satoh, N., Queinnec, E., Ereskovsky, A., Lapebie, P., Corre, E., Delsuc, F., King, N., Worheide, G. & Manuel, M. (2017) A large and consistent phylogenomic dataset supports sponges as the sister group to all other animals. Current Biology, 27, 958-967. https://doi.org/10.1016/j.cub.2017.02.031
  • Song, F., Li, H., Jiang, P., Zhou, X., Liu, J., Sun, C., Vogler, A.P. & Cai, W. (2016) Capturing the phylogeny of Holometabola with mitochondrial genome data and Bayesian site-heterogeneous mixture models. Genome Biology and Evolution, 8, 1411- 1426. https://doi.org/10.1093/gbe/evw086
  • Tillyard, R.J. (1935) The evolution of the scorpion-flies and their derivatives (order Mecoptera). Annals of the Entomological Society of America, 28, 1-45. https://doi.org/10.1093/aesa/28.1.1
  • Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics, 27, 171-180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  • Wheeler, W.C., Whiting, M., Wheeler, Q.D. & Carpenter, J.M. (2001) The phylogeny of the extant hexapod orders.Cladistics, 17, 113-169. https://doi.org/10.1111/j.1096-0031.2001.tb00115.x
  • Whiting, M. (2003) Phylogeny of the holometabolous insects. The most successful group of terrestrial organisms. In: Cracraft, J. & Donghue, M. (Eds). Assembling the Tree of Life. Oxford University Press, Oxford, pp. 345-364.
  • Whiting, M., Whiting, A.S. & Hastriter, M.W. (2003) A comprehensive phylogeny of Mecoptera and Siphonaptera. Entomologische Abhandlungen, 61, 169.
  • Whiting, M.F. (2002a) Mecoptera is paraphyletic: multiple genes and phylogeny of Mecoptera and Siphonaptera. Zoologica Scripta, 31, 93-104. https://doi.org/10.1046/j.0300-3256.2001.00095.x
  • Whiting, M.F. (2002b) Phylogeny of the holometabolous insect orders: molecular evidence. Zoologica Scripta, 31, 3-15. https://doi.org/10.1046/j.0300-3256.2001.00093.x
  • Whiting, M.F., Carpenter, J.C., Wheeler, Q.D. & Wheeler, W.C. (1997) The Strepsiptera problem: phylogeny of the holometabolous insect orders inferred from 18S and 28S ribosomal DNA sequences and morphology. Systematic Biology, 46, 1-68. https://doi.org/10.1093/sysbio/46.1.1
  • Whiting, M.F., Whiting, A.S., Hastriter, M.W. & Dittmar, K. (2008) A molecular phylogeny of fleas (Insecta: Siphonaptera): origins and host associations. Cladistics, 24, 677-707. https://doi.org/10.1111/j.1096-0031.2008.00211.x
  • Wiegmann, B.M., Trautwein, M.D., Kim, J.-W., Cassel, B.K., Bertone, M.A., Winterton, S.L. & Yeates, D.K. (2009) Single-copy nuclear genes resolve the phylogeny of the holometabolous insects. BMC Biology, 7, 34. https://doi.org/10.1186/1741-7007-7-34
  • Wiegmann, B.M., Trautwein, M.D., Winkler, I.S., Barr, N.B., Kim, J.-W., Lambkin, C., Bertone, M.A., Cassel, B.K., Bayless, K.M., Heimberg, A.M., Wheeler, B.M., Peterson, K.J., Pape, T., Sinclair, B.J., Skevington, J.H., Blagoderov, V., Caravas, J., Kutty, S.N., Schmidt-Ott, U., Kampmeier, G.E., Thompson, F.C., Grimaldi, D.A., Beckenbach, A.T., Courtney, G.W., Friedrich, M., Meier, R. & Yeates, D.K. (2011) Episodic radiations in the fly tree of life. Proceedings of the National Academy of Sciences, 108, 5690-5695. https://doi.org/10.1073/pnas.1012675108
  • Wille, A. (1960) The phylogeny and relationships between the insect orders. Revista de biologia tropical, 8, 93-123.
  • Willmann, R. (2003) Die phylogenetischen beziehungen der Insecta: offene fragen und probleme. Verhandlungen Westdeutscher Entomologentag, 2001, 1-64.
  • Wood, D.M. & Borkent, A. (1989) Phylogeny and classification of the Nematocera. In: McAlpine, J.F. & Wood, D.M. (Eds). Manual of Nearctic Diptera. Vol. 3. Research Branch, Agriculture Canada, Ottawa, pp. 1333-1370.
  • Yoshizawa, K. & Johnson, K.P. (2010) How stable is the "polyphyly of lice" hypothesis (Insecta: Psocodea)? A comparison of phylogenetic signal in multiple genes. Molecular Phylogenetics and Evolution, 55, 939-951. https://doi.org/10.1016/j.ympev.2010.02.026
  • Zhao, X., Wang, B., Bashkuev, A.S., Aria, C., Zhang, Q., Zhang, H., Tang, W. & Engel, M.S. (2020) Mouthpart homologies and life habits of Mesozoic long-proboscid scorpionflies. Science Advances, 6, eaay1259. https://doi.org/10.1126/sciadv.aay1259