Published October 28, 2020 | Version v1

Map-projected time-lapse of zebrafish left-right somite formation

  • 1. EPFL, Lausanne

Description

Zebrafish embryos in their chorions were imaged from 6 angles in a multiview light sheet microscope. The imaging was started 10 hours post fertilization and was performed for about 4 to 5 hours at a frame interval of 5 min. The resulting images were fused using FIJI, followed by nuclei detection in the fused images and map projection. This data set contains map projected time-lapses of 6 utr::mcherry transgenic embryos. Utrophin binds to actin filaments and was used as a somite boundary marker. The pixel size varies spatially in a map as well as across different projection layers in a single time-lapse. The corresponding Map_pixel_size mat file for each time-lapse contains respective pixel sizes.

Files

Files (48.9 GB)

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Additional details

Related works

Is documented by
Preprint: 10.1101/2020.08.14.251645 (DOI)

Funding

Wellcome Trust
Patterning embryos with genetic oscillations. 098025