Global Characterization of Megakaryocytes in Bone Marrow, Peripheral Blood, and Cord Blood by Single-cell RNA Sequencing
Description
Megakaryocytes (MK) are mainly derived from bone marrow (BM) and are mainly involved in platelet production. Recent studies have shown that MK derived from BM may have immune function, and that MK from peripheral blood (PB) are associated with prostate cancer. We analyzed more than 1.2 million single-cell transcriptome data from 132 samples of PB, BM, and cord blood (CB) from healthy individuals and patients, and obtained 4474 MK single cell and 14 MK subtypes. We found that MK were widely distributed and the amount of MK in PB was more than that in BM and there were specificity MK subtypes in PB. We found classical MK1 with typical MK characteristics and non-classical MK2 closely related to immunity which was the most common subtype in BM and CB. Classical MK1 was closely related to Non-Small Cell Lung Cancer (NSCLC) and has diagnostic ability. MK2 may have potential adaptive immune function and play a role in tumor NSCLC and autoimmune diseases Systemic Lupus Erythematosus. This study deepened our understanding of MK and suggested that MK had potential immune functions and was involved in various diseases.
Notes
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 81800195
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 81460315
Funding provided by: Key Clinical Projects of Peking University Third Hospital*
Crossref Funder Registry ID:
Award Number: BYSYZD2019026
Funding provided by: Interdisciplinary Medicine Seed Fund of Peking University
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100012487
Award Number: BMU2018MB004
Funding provided by: Natural Science Foundation of Beijing Municipality
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100004826
Award Number: 7132183
Funding provided by: Natural Science Foundation of Beijing Municipality
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100004826
Award Number: 7182178
Funding provided by: China Health Promotion Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100017661
Award Number: CHPF-zlkysx-001
Funding provided by: Scientific Research Foundation from Health Commission of Jiangxi Province, China*
Crossref Funder Registry ID:
Award Number: 20141114
Funding provided by: Science and Technology Research Foundation from Educational Commission of Jiangxi Province, China*
Crossref Funder Registry ID:
Award Number:
Funding provided by: Science and Technology Research Foundation from Educational Commission of Jiangxi Province, China*
Crossref Funder Registry ID:
Award Number: GJJ14676
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