Fish mock community with 41 species from 13 orders

Tissue extracts of 41 North American fish species were obtained from the Ministère des Forêts, de la Faune et des Parcs (Québec). The selected species were chosen to represent a diversity of families within the Actinopterygii, and to include some species known to be present at the Experimental Lakes Area. Muscle or fin tissue was extracted using Qiagen Blood and Tissue kits and equimolarised to 15ng/µl. Library preparation and next-generation sequencing (NGS) was performed by equimolarising the DNA and combining two replicate mock community libraries which were then PCR amplified and sequenced. Sequencing was conducted using 2x300bp Illumina MiSeq at the McGill University and Génome Québec Innovation Centre, Montréal.