Published July 23, 2020 | Version v1
Dataset Open

Catalysis of L-cysteine by IscS in the presence of IscU (dataset formatted for analysis by interferENZY)

Authors/Creators

  • 1. i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto

Contributors

  • 1. i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto
  • 2. LEPABE – Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto
  • 3. Maurice Wohl Clinical Neuroscience Institute, King's College London
  • 4. ICBAS – Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto

Description

Main description

This dataset depicts the catalysis of L-cysteine to sulfur by the desulfurase IscS in the presence of the scaffold protein IscU. This kinetic system was described in the PhD thesis "New tools for enzymatic characterization and inhibitor screening: post-Michaelis-Menten kinetic analysis" by Maria Filipa Pinto (https://hdl.handle.net/10216/126202), and this dataset was then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis.

 

Dataset organization

Line 1: Tab-separated initial concentrations of substrate L-cysteine in micromolar (µM) concentration

Line 2: Concentration of enzyme (1 µM)

Line 3: Units of time

Line 4: Units of concentration for substrate values and measurements

Line 5: Dataset name

Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1

 

Contact information:

Maria Filipa Pinto (mfpinto@i3s.up.pt)
Pedro M. Martins (pmartins@ibmc.up.pt)

i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: +351 226 074 900

Notes

This work was financially supported by: (i) PhD fellowship SFRH/BD/109324/2015, FCT – Fundação para a Ciência e Tecnologia; (ii) Doutoramento em Ciências Biomédicas – Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto (ICBAS-UP); (iii) FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES) in the framework of the projects POCI-01-0145-FEDER-031323 (PTDC/MED-FAR/31323/2017), POCI-01-0145-FEDER-031173 (PTDC/BIA-BFS/31173/2017), POCI-01-0145-FEDER-007274 ("Institute for Research and Innovation in Health Sciences") and POCI-01-0145-FEDER-006939 (Laboratory for Process Engineering, Environment, Biotechnology and Energy); (iv) Project UID/EQU/00511/2019 – Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE funded by national funds through FCT/MCTES (PIDDAC); (v) FEDER through Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement in the framework of Projects Norte-01-0145-FEDER-000008 and "LEPABE-2-ECO-INNOVATION" – NORTE-01-0145-FEDER-000005.

Files

interferENZY_IscSIscU.txt

Files (19.0 kB)

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md5:6f2c922fc3dd9dfc006e14181e1669e3
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Additional details

Related works

Is derived from
Thesis: 10216/126202 (Handle)
Is supplement to
Software: 10.5281/zenodo.3956410 (DOI)

References

  • Pinto, M. F. (2020). New tools for enzymatic characterization and inhibitor screening: post-Michaelis-Menten kinetic analysis [Doctoral Thesis], Universidade do Porto, Porto, Portugal