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Published July 4, 2020 | Version v1.0.1
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Data collection for Tsuji et al., 2020, Anoxygenic phototrophic Chloroflexota member uses a Type I reaction center

  • 1. University of Waterloo
  • 2. Tokyo Metropolitan University
  • 3. Wilfrid Laurier University
  • 4. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH

Description

Supplementary data files associated with Tsuji et al., 2020, "Anoxygenic phototrophic Chloroflexota member uses a Type I reaction center". These files are used by code in a corresponding GitHub repository (https://github.com/jmtsuji/Ca-Chlorohelix-allophototropha-RCI) that shows how various analyses that are presented in the paper were conducted.

Files included:

  • Capt_S15_sequencer_data_raw.tar.gz -- Gzipped tarball containing the raw Illumina MiSeq output data for the 'Candidatus Chlorohelix allophototropha' subculture 15 sequencing run. The run represents a read cloud sequencing run relying on TELL-Seq technology. Indices can be parsed directly from raw output data using the Tell-Read pipeline.
  • scaffold.full.fasta.gz -- the assembled scaffolds generated using Tell-Read and Tell-Link on the above raw MiSeq output data.
  • Ca_Chx_allophototropha_L227-S17_prokka_ORFs.faa.gz and Ca_Chloroheliaceae_bin_L227_5C_prokka_ORFs.faa.gz -- predicted open reading frames (ORFs) from the curated genomes of 'Candidatus Chlorohelix allophototropha' and 'Candidatus Chloroheliales bin L227-5C', respectively. These ORFs were predicted using prokka and were used for the analyses presented in the paper. They are similar to, but differ somewhat from, the ORFs predicted by the NCBI gene annotation pipeline that was used upon uploading the genomes to the NCBI Genbank database. Thus, these original ORF files are provided for reference in case comparison is ever needed to the publicly accessible Genbank files.
  • I_TASSER_homology_models_full_output.tar.gz -- Gzipped tarball containing the full output from I-TASSER for homology models of key phototrophy-related genes encoded by 'Candidatus Chlorohelix allophototropha' and 'Candidatus Chloroheliales bin L227-5C'. After unpacking the tarball, view a summary of the I-TASSER output for each gene by clicking on the 'index.html' file in that gene's folder.

Notes

Changelog: - v1.0.0: First version - v1.0.1: Added uncurated genome bin files

Files

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