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Published July 2, 2020 | Version 1.2.0
Software Open

nf-core/chipseq: nf-core/chipseq v1.2.0 - Platinum Fox

Authors/Creators

  • 1. The Francis Crick Institute
  • 2. National Genomics Infrastructure, Science for Life Laboratory
  • 3. Science for Life Laboratory
  • 4. Boehringer Ingelheim
  • 5. University of Miami
  • 6. @SciLifeLab | Karolinska Institutet

Description

[1.2.0] - 2020-07-02 Added
  • #138 - Add social preview image
  • #153 - Add plotHeatmap
  • #159 - expose bwa mem -T parameter
  • nf-core/atacseq#63 - Added multicore support for Trim Galore!
  • nf-core/atacseq#75 - Include gene annotation versions in multiqc report
  • nf-core/atacseq#76 - featureCounts coupled to DESeq2
  • nf-core/atacseq#79 - Parallelize DESeq2
  • nf-core/atacseq#97 - PBC1, PBC2 from pipeline?
  • nf-core/atacseq#107 - Add options to change MACS2 parameters
  • Regenerated screenshots and added collapsible sections for output files in docs/output.md
  • Update template to tools 1.9
  • Replace set with tuple and file() with path() in all processes
  • Capitalise process names
  • Parameters:
    • --bwa_min_score to set minimum alignment score for BWA MEM
    • --macs_fdr to provide FDR threshold for MACS2 peak calling
    • --macs_pvalue to provide p-value threshold for MACS2 peak calling
    • --skip_peak_qc to skip MACS2 peak QC plot generation
    • --skip_peak_annotation to skip annotation of MACS2 and consensus peaks with HOMER
    • --skip_consensus_peaks to skip consensus peak generation
    • --deseq2_vst to use variance stabilizing transformation (VST) instead of regularized log transformation (rlog) with DESeq2
    • --publish_dir_mode to customise method of publishing results to output directory nf-core/tools#585
Removed
  • --tss_bed parameter
Fixed
  • #118 - Running on with SGE
  • #132 - BigWig Error: sort: cannot create temporary file in '': Read-only file system
  • #154 - computeMatrix.val.mat.gz files not zipped
  • nf-core/atacseq#71 - consensus_peaks.mLb.clN.boolean.intersect.plot.pdf not generated
  • nf-core/atacseq#73 - macs_annotatePeaks.mLb.clN.summary.txt file is not created
  • nf-core/atacseq#86 - bug in the plot_homer_annotatepeaks.r script
  • nf-core/atacseq#102 - Incorrect Group ID assigned by featurecounts_deseq2.r
  • nf-core/atacseq#109 - Specify custom gtf but gene bed is not generated from that gtf?
  • Make executables in bin/ compatible with Python 3
Dependencies
  • Add bioconductor-biocparallel 1.20.0
  • Add markdown 3.2.2
  • Add pigz 2.3.4
  • Add pygments 2.6.1
  • Add pymdown-extensions 7.1
  • Add python 3.7.6
  • Add r-reshape2 1.4.4
  • Add r-tidyr 1.1.0
  • Update bedtools 2.27.1 -> 2.29.2
  • Update bioconductor-deseq2 1.20.0 -> 1.26.0
  • Update bioconductor-vsn 3.46.0 -> 3.54.0
  • Update deeptools 3.2.1 -> 3.4.3
  • Update fastqc 0.11.8 -> 0.11.9
  • Update gawk 4.2.1 -> 5.1.0
  • Update homer 4.9.1 -> 4.11
  • Update macs2 2.1.2 -> 2.2.7.1
  • Update multiqc 1.7 -> 1.8
  • Update phantompeakqualtools 1.2 -> 1.2.2
  • Update picard 2.19.0 -> 2.23.1
  • Update pysam 0.15.2 -> 0.15.3
  • Update r-base 3.4.1 -> 3.6.2
  • Update r-ggplot2 3.1.0 -> 3.3.2
  • Update r-lattice 0.20_35 -> 0.20_41
  • Update r-optparse 1.6.0 -> 1.6.6
  • Update r-pheatmap 1.0.10 -> 1.0.12
  • Update r-scales 1.0.0 -> 1.1.1
  • Update r-upsetr 1.3.3 -> 1.4.0
  • Update r-xfun 0.3 -> 0.15
  • Update samtools 1.9 -> 1.10
  • Update subread 1.6.4 -> 2.0.1
  • Update trim-galore 0.5.0 -> 0.6.5
  • Update ucsc-bedgraphtobigwig 377 -> 357

Files

nf-core/chipseq-1.2.0.zip

Files (1.0 MB)

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md5:26b443afcdf7fd9a8f0ce4149f402771
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Additional details

Related works

Is supplement to
https://github.com/nf-core/chipseq/tree/1.2.0 (URL)