DNMT3B -579G>T POLYMORPHİSM AND THE RİSK OF COLORECTAL CANCER IN AZERBAİJAN POPULATİON

BACKGROUND

DNA methylation is one of the important mechanisms for epigenetic modifications and under the control of DNA methyltransferases (DNMTs). The family of DNMTs consists of five different enzymes (DNMT1, DNMT2, DNMT3A, DNMT3B, and DNMT3L). The purpose of this case-control study was to determine the association between DNMT3B −579 G>T polymorphism and the risk of colorectal cancer in the Azerbaijan population.

MATERİALS AND METHODS

Blood samples were collected from the Center of Scientific Surgery Named After Academician Topchubashov. One hundred and fifteen patients with colorectal cancers and 115 controls were included in the study. Genomic DNA isolation was performed with the Qiagen kit (QIAamp DNA Mini Kit) in the Human Genetics Laboratories of the Institute of Genetic Resources. The genotyping of DNMT3B -579G>T was performed by PCR-RFLP.

RESULTS

The frequencies of the GG, GT, and TT genotypes of DNMT3B were 41.7%, 40%, and 18.3% in the patients with colorectal cancer and 28.7%, 54.8%, and 16.5% in the healthy control group respectively. The GT genotype was significantly different between patients with colorectal cancer and control subjects. Mutant TT genotype was more frequent in the patient group compared to the control group.

CONCLUSİON

Our findings suggest that DNMT3B -579G>T polymorphism represents a genetic risk factor that may play an important role in colorectal cancer development.