Published April 8, 2017 | Version v1
Software Open

GUV membrane linearization macro

  • 1. Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany; International Max Planck Research School for Molecular Biology at the University of Göttingen, Germany

Description

GUV membrane linearization macro v1.0.0
http://dx.doi.org/10.5281/zenodo.376618

This package contains:
GUV_membrane_linearization_macro.ijm

This macro was developed by Agata Witkowska for the study
Witkowska A. & Jahn R., Biophysical Journal (2017)
"Rapid SNARE-mediated fusion of liposomes and chromaffin granules with giant unilamellar vesicles"
http://dx.doi.org/10.1016/j.bpj.2017.03.010

This Fiji macro can be used for automatic detection of GUV on a microscopy image (1 GUV on image present), linearization of its membrane with a polar transformation method, removal of saturated pixels and measurement of peak membrane intensities on a transformed image. It returns GUV diameter, mean membrane peak intensity and std of it.
Required plugin: Polar Transformer.

Files

GUV_membrane_linearization_macro.zip

Files (2.2 kB)

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Additional details

Related works

Is supplement to
10.1016/j.bpj.2017.03.010 (DOI)