Published April 8, 2020
| Version v1
Dataset
Open
Highly multiplexed histology reveals phenotypic and spatial characteristics of human Innate Lymphoid Cells in chronic inflammation - MELC colon data-set
Authors/Creators
- 1. DRFZ and Immune Dynamics, Rheumatology an Clinical Immunology, Charité - Universitätsmedizin Berlin
Description
50 marker MELC Run in human colon. Each image shows the same field of view, sequentially stained with the depicted fluorescence-labelled antibodies, including surface proteins and transcription factors. Images contain 2024 x 2024 pixels and are generated using an inverted wide-field fluorescence microscope with a 20x objective, a lateral resolution of 325 nm and an axial resolution above 5 µm. Images have not been normalized and intensities have not been adjusted.
Notes
This study was supported by funding from the Deutsche Forschungsgemeinschaft, (SPP1937, HA5354/8-2 to A.E.H.) The work was supported by an "Adding 3R Value" grant of the Charité - Universitätsmedizin, Berlin.
Files
CD127-PE_450_450_111_009.tiff
Files
(419.4 MB)
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Additional details
Related works
- Is supplement to
- Dataset: 10.5281/zenodo.3744152 (DOI)