Published February 24, 2020 | Version V1.0
Technical note Open

CRISPR-mediated introduction of single nucleotide changes in iPSCs

  • 1. MNI, McGill University


This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.


EDDU-009-01_CRISPR editing of iPSCs_v1.0.pdf

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