Confocal microscopy image stacks from "Temporal integration of auxin information for the regulation of patterning"

This dataset contains raw images in CZI format (Zeiss) of shoot apical meristems (SAM) from Arabidopsis thaliana transgenic lines qDII-pCLV3-pDR5 or qDII-pCLV3-PIN1. See (Galvan-Ampudia and Cerutti et al.) for detailed information. This data constitutes the input of the sam_spaghetti pipeline (https://gitlab.inria.fr/mosaic/publications/sam_spaghetti) and can be processed using the scripts and examples provided in the package.

File information:

File names containing qDII-CLV3-DR5 have the following data:

• Channel 1: DII-VENUS-N7
• Channel 2: pDR5:2xmTurquoise2
• Channel 3: pRPS5a:TagBFP-SV40
• Channel 4: pCLV3:mCherry-N7

File names containing qDII-CLV3-PIN1-PI have the following data:

• Channel 1: DII-VENUS-N7
• Channel 2: pPIN1:PIN1-GFP
• Channel 3: Propidium Iodide (cell walls)
• Channel 4: pRPS5a:TagBFP-SV40
• Channel 5: pCLV3:mCherry-N7

Time-lapse sequences are identified as follows:

• qDII-CLV3-DR5-E27-LD-SAM7.czi
• qDII-CLV3-DR5-E27-LD-SAM7-T5.czi
• qDII-CLV3-DR5-E27-LD-SAM7-T10.czi

where:

• qDII-CLV3-DR5 indicates the line
• E$$-LD (e.g. E25-LD, E27-LD, etc) indicates independent biological replicas
• SAM$ is the meristem (technical replica)
• T$ indicates the time elapsed after the first image (in hours)

For example qDII-CLV3-DR5-E27-LD-SAM7-T5.czi is an image of the 7th SAM of the set E27, acquired 5 hours after the first image.