Data for marine fungi engineered to convert D-galacturonic acid to galactaric acid

Two Excel files are stored, related to the successful engineering of two marine fungi (Trichoderma sp. LF328 and Coniochaeta sp. MF729) for the production of galactaric (mucic) acid from D-galacturonic acid, which is described in Vidgren et al. 2020. One file contains the HPLC data for cultivations carried out in 24-well plates with medium containing D-galacturonic acid, poly-D-galacturonic acid or pectin as a source of D-galacturonic acid, with lactose, xylose, glucose or glycerol as co-substrates. Cultures were sampled on days 3, 4 and 6, and sometimes on days 5 and 7, as indicated in the data. Data for negative results (e.g. lack of consumption of galactaric acid by the parent strains) is also included.

The second file contains the HPLC data for cultivations carried out in 1 (Coniochaeta sp. C1) or 1.5 L (Trichoderma sp. T2) bioreactors, with information on culture volume and product yield for Trichoderma sp. T2.