During a survey of helminth parasites of the brown ground snake, Atractus major Boulenger, 1894 (Serpentes: Dipsadidae) from Moyobamba, region of San Martin (northeastern Peru), a new species of Glossidiella Travassos, 1927 (Plagiorchiida: Plagiorchiidae) was found and is described herein based on morphological and ultrastructural data. The digeneans found in the lung were measured and drawings were made with a drawing tube. The ultrastructure was studied using scanning electron microscope. Glossidiella peruensis sp. nov. is easily distinguished from the type- and only species of the genus, Glossidiella ornata Travassos, 1927, by having an oblong cirrus sac (claviform in G. ornata), distinctly ovate testes (rounded testes in G. ornata) and button-like papillae on the dorsal edge of the oral sucker region (absent in G. ornata). In addition, G. peruensis sp. nov. differs from G. ornata by possessing a longer distance between testes and substantially wider oral and ventral suckers. This is the first time that a species of digenean is described and reported parasitizing snakes in Peru.