Published January 9, 2020 | Version v1
Journal article Open

PROTEIN PROFILE OF SOMATIC ANTIGENS OF PARAMPHISTOMUM CERVI

Authors/Creators

Description

Paramphistomum cervi is one of the most common trematode parasites infecting ruminants across the globe. The current study was carried out to identify the somatic antigens present in P. cervi using SDS PAGE. The electrophoretic pattern of somatic proteins of P. cervi under reducing conditions in 12% SDS revealed the presence of several bands with the molecular weights ranging between 10-150 kDa. The major bands appeared at 10, 18, 23, 40, 50, 75 kDa. The proteins can be subjected to further experimental analysis for detection of their possible role in serodiagnosis or vaccine development.

Files

6. IJZAB ID No. 484.pdf

Files (392.9 kB)

Name Size Download all
md5:8fe187d505f2718d89894950d04a4147
392.9 kB Preview Download

Additional details

References

  • Anuracpreeda, P., Poljaroen, J., Chotwiwatthanakun, C., Tinikul, Y., & Sobhon, P. (2013). Antigenic components, isolation and partial characterization of excretion secretion fraction of Paramphistomum cervi. Experimental Parasitology, 133(3), 327-333.
  • Arora, R., Singh, N., Juyal, P., & Ghosh, S. (2010). Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum. Journal of Parasitic Diseases, 34(1), 57-61.
  • Eslami, A., Taefie-Nasrabadi, N., & Jalosian, F. (2012). Variations to somatic protein profiles of Dicrocoelium dendriticum in Iranian ruminants. Iranian Journal of Veterinary Medicine, 6(2), 95-98.
  • E. (2008). Protein Profile of Fasciola gigantica Antigens. The Sudan Journal of Vetenary Research. 23, 1-9.
  • Gul, N., Tak, H., Fazilli, K.M., Abdullah, I., & Sofi, T.A. (2017). Prevalence of Fasciola Infection in Slaughtered Animals in Kashmir. International Journal of Veterinary Sciences and Animal Husbandry, 1(2), 30-36.
  • Jaiswal, A.K., Shanker, D., Sudan, V., & Singh, A. (2018). Diagnostic potential of low molecular weight excretory secretory proteins of Paramphistomum epiclitum for caprine amphistomosis. Veterinary Parasitology, 257, 5-9.
  • Jyoti, A.; Prasad, and Singh, N.K. (2014). Evaluation of Antibody Response to Various Developmental Stage Specific Somatic Antigens of Paramphistomum epiclitum in Goats. BioMed Research International.1-6
  • Latchumikanthan, A., Soundararajan, C., Dhinakarraj, G., & Basith, S.A. (2012). Purification and characterization of excretory/secretory antigens of Fasciola gigantica. Tamilnadu Journal of Vetenary and Animal Science, 8(1), 14-18.
  • Lefevre, P.C., Blancou, J., & Chermette, R. (2003). Principales maladies infectieuses et parasitaires du bétail: Europe et régions chaudes. Journal of Agriculture Organization,1-3.
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J.(1951). Protein measurement with the Folin phenolreagent. Journal of Biological Chemistry, 193, 265-275.
  • Meshgi, B., Eslami, A., & Halajian, A. (2009). Determination of diagnostic antigens in cattle amphistomiasis using western blotting. Iranian Journal of Parasitology, 32-37.
  • Meshgi, B., Eslami, A., & Hematzadeh, F. (2008). Determination of somatic and excretory-secretory antigens of Fasciola hepatica and Fasciola gigantic using SDS-page. Iranian Journal of Veterinary Research, Shiraz University, 9(1),1-22.
  • Rolfe, P., Boray, J., & Collins, G. (1994). Pathology of infection with Paramphistomum ichika wai in sheep. International Journal for Parasitology, 24(7), 995-1004.
  • Salib, F.A., Halium, M.M.A., Mousa, W.M., & Massieh, E. S.A. (2015). Evaluation of indirect ELISA and Western blotting for the diagnosis of amphistomes infection in cattle and buffaloes. International Journal of Livestock Research, 5(3), 71-81.
  • Yamaguti, S. (1958). Systema helminthum: the digenetic trematodes of vertebrates. Volume 1, Newyork: Interscience publisher

Subjects

Zoology
10.5281/zenodo.3602582