Digital Holographic Microscopy and Python

Holograms are Interference, circular pattern of bright and dark lines surrounding an object due to light passage over it in 2D plane. The Hungarian-British physicist Dennis Gabor was awarded with Nobel Prize for inventing and applying this phenomenon for improving electron microscopy in 1971. These patterns contains 3 dimensional information of the object and computationally possible to reconstruct/simulate. Gives a method to image micrometer size tiny objects such as microorganism or bio molecules without any optical lenses and has immense application in low cost, lens less microscopy.

Holopy, a python package for inference method of simulating 3d information from digital holograms. Objective of the poster is to introduce the holopy and demonstrate 3d simulation of 2-5 micrometer size particles from holographic image using Python code base. It also intended to explore the application of holographic microscopy combined with machine learning for air pollution monitoring.