Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in chordates and vertebrates with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then “binned” together based on genomic location. Each bin is then annotated with miRBase mature sequences and hairpins, as well as other types of RNA obtained from Ensembl’s Biomart.