Cdc42 couples T cell receptor endocytosis to GRAF1-mediated tubular invaginations of the plasma membrane

This Dataset contains primary data used for the publication "Cdc42 couples T cell receptor endocytosis to GRAF1-mediated tubular invaginations of the plasma membrane" published online on 04. November 2019
doi:10.3390/cells8111388

Abstract: T cell activation is immediately followed by internalization of the T cell receptor (TCR).
TCR endocytosis is required for T cell activation, but the mechanisms supporting removal of TCR
from the cell surface remain incompletely understood. Here we report that TCR endocytosis is
linked to the clathrin-independent carrier (CLIC) and GPI-enriched endocytic compartments
(GEEC) endocytic pathway. We show that unlike the canonical clathrin cargo transferrin or the
adaptor protein Lat, internalized TCR accumulates in tubules shaped by the small GTPase Cdc42
and the Bin/amphiphysin/Rvs (BAR) domain containing protein GRAF1 in T cells. Preventing
GRAF1-positive tubules to mature into endocytic vesicles by expressing a constitutively active
Cdc42 impairs the endocytosis of TCR, while having no consequence on the uptake of transferrin.
Together, our data reveal a link between TCR internalization and the CLIC/GEEC endocytic route
supported by Cdc42 and GRAF1.

Data are organised in compressed (.zip) folders entitled as the corresponding Figures in the publication.

Programs we recommend to view the files are:
.fcs files:     FlowJo software v10 (Tree Star, Ashland, OR, USA)
.lif files:       LAS X v3 (Leica Microsystems, Wetzlar, Germany)
.pzfx files:   Prism v7 software (GraphPad, San Diego, CA, USA)

In case this Dataset is updated, new version will be available with doi:10.5281/zenodo.3545842