Supplementary multimedia files Hao M et al.

Multimedia movie 3               Live calcium imaging of myenteric neurons in adult small intestine during electrical stimulation of villus tip. The movie is 60 sec in duration, captured at 2 Hz. Focal electrical stimulation is applied at 10 sec. Increases in [Ca²⁺]_i can be observed in 3 cells.

Multimedia movie 4               Live calcium imaging of myenteric neurons in E15.5 small intestine during electrical stimulation of villus tip. The movie is 60 sec in duration, captured at 2 Hz. Focal electrical stimulation is applied at 10 sec. Many more cells and ganglia are visible in each field of view. Increases in [Ca²⁺]_i can be observed in several cells.

Multimedia movie 5                 Live calcium imaging of myenteric neurons in adult small intestine during pressurised injection of 5-HT into a villus tip. The movie is 60 sec in duration, captured at 2 Hz. 5-HT is injected at 10 sec. Increases in [Ca²⁺]_i can be observed in several cells.

Multimedia movie 6                 Live calcium imaging of myenteric neurons in P7 small intestine during pressurised injection of 5-HT into a villus tip. The movie is 60 sec in duration, captured at 2 Hz. 5-HT is injected at 10 sec. Increases in [Ca²⁺]_i can be observed in several cells.