Published October 15, 2019 | Version v1
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Genetic Diversity Corylus avellana L. in Lesser Caucasus of Azerbaijan

Authors/Creators

  • 1. Azerbaijan State Agrarian University

Description

The article is devoted to the results of a study of the genetic polymorphism of hazel (Corylus avellana), growing in the Lesser Caucasus within Azerbaijan. For the analyzes used nuclear DNA extracted from sheet material. DNA extraction, PCR and ISSR analyzes were carried out according to standard methods (CTAB, PCR, ISSR protocols). According to the results of analyzes using 4 ISSR primers, the number of identified fragments was 42, which corresponds to 9–12 loci per primer (~10.8). Of the 42 fragments identified, 34 (80.95%) are polymorphic, and 8 (19.05%) are monomorphic. The number of polymorphic loci varies in the range of 7–10. With the smallest number of amplified loci in the UBC811 primer, the largest number of them occurs in the UBC827 primer. Depending on the primer, the number of amplified polymorphic loci varies within 63–90%. The level of ISSR primer polymorphism is on average 86% (75–96%). The average value of the actual heterozygosis (H0) is 0.359, and the expected heterozygosis (HE) is 0.414. According to the results of the cluster analysis, 70 hazel genotypes are combined in 9 clusters. Despite the fact that the populations are remotely and orographically sufficiently isolated, which excludes the flow of genetic information between them, the results of the cluster analysis show that genotypes from different populations are combined into a common sub-cluster in terms of genetic similarity. This is due to the common origin of hazel in populations. In the distant past, this species was represented by an extensive common range, which was fragmented as a result of geological processes. The homogeneous disjunction of the continuous range occurred.

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Journal article: 2414-2948 (ISSN)
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Journal article: http://www.bulletennauki.com/47-05 (URL)

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