Catalase restores the altered mRNA expression of collagen and matrix metalloproteinases by dermal fibroblasts exposed to reactive oxygen species

We investigated the effects of reactive oxygen species (ROS) on mRNA expression of proalphal (l) collagen, proalphal (lll) collagen, matrix metalloproteinases-1 (MMP-1), 72 kDa type lV collagenase (MMP-2), and tissue inhibitor of metalloproteinase (TIMP-1) by normal human dermal fibroblasts in a novel three-dimensional culture. Fibroblasts exposed to ROS generated by the hypoxanthine-xanthine oxidase system revealed an increased mRNAexpression of MMP-1 and MMP-2 with a maximum at48 h and 72h after
exposure. A slight increase in the mRNA level of tissue inhibitor of metalloproteinase (TIMP-1) was observed.