Published May 26, 2019 | Version v2
Dataset Open

Systematic assessment of burst impurity in confocal-based single-molecule fluorescence detection using Brownian motion simulations - photon timetag simulation files

  • 1. The Hebrew University of Jerusalem

Contributors

  • 1. The Hebrew University of Jerusalem

Description

Attached are the photon timestamp and channels simulated for different 3D diffusing molecules simulations at different conditions (simulation was performed by PyBroMo).

Each of the files has, in its name, a code. The meaning of the codes are as following:

f32445 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model 
a01f8f - 15 molecules at a concentration of 31 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model
9ff667 - 15 molecules at a concentration of 15.5 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model
71154a - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 22.5 micron^2/s - 60 second simulation using a numerical PSF model
ad926d - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 60 second simulation using a numerical PSF model
1ab235 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 180 second simulation using a numerical PSF model
d00978 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 180 second simulation using a numerical PSF model

 

2469bb - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model 
4be121 - 15 molecules at a concentration of 31 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model
a7088f - 15 molecules at a concentration of 15.5 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model
023983 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 22.5 micron^2/s - 60 second simulation using a Gaussian PSF model
653f61 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 60 second simulation using a Gaussian PSF model
4f06ee - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 180 second simulation using a Gaussian PSF model
dec32c - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 180 second simulation using a Gaussian PSF model

 

85b0a1 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 60 second simulation using a Numerical PSF model
964ef3 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 180 second simulation using a Numerical PSF model

 

f28f6e - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 60 second simulation using a Gaussian PSF model
c311dd - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 180 second simulation using a Gaussian PSF model

Files

Files (104.4 MB)

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