Enzymes from the BRENDA database annotated with organism growth temperatures

Experimental as well as predicted organism growth temperatures were used to annotate enzymes from the BRENDA database (doi: 10.1093/nar/gky1048, https://www.brenda-enzymes.org) version 2018.1. The growth temperature annotation can be used as an estimate of the enzymes catalytic optima.

The "enzyme_to_growth_temp_mapping.tsv" file is a tab-separated file with the data headers: ec, uniprot_id, domain, organism, source and growth_temp. The ec column lists enzyme classes. The uniprot_id lists UniProt identifiers. The domain column lists the domain of life (superkingdom), either Archaea, Bacteria, or Eukaryota. The organism column lists organism names, with strain designations removed and formatted to lowercase characters with an underscore _ separating the name parts. The source column lists whether experimental growth temperatures or predicted ones were used for the annotation. The growth_temp column lists, in degrees centigrade, the organism growth temperature.

The "all_enzyme_sequences.fasta" file follows the standard FASTA format and contains the protein sequences for all annotated enzymes. UniProt identifiers is used as a header for each of the sequences.