Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk

E. coli is a significant pathogen that contributes to the genesis of both human and animal diseases. 20 different E. coli isolates were found in milk, stool, and utensil samples. Depending on whether each E. coli strain has the gene, the chuA, and the DNA segment TspE4.C2 has joined. the intestinal groups A and B1 as well as the extraintestinal groups B2 and D. The findings of the PCR-based analysis revealed that the B2 3 subgroup contained 6 feces samples, 4 milk samples and 2 utensil samples, where these groups are separated into six subgroups (A0, A1, B23, D1, D2). While one stool sample, three milk samples, and one utensil sample were D2 subgroup members, two stool samples and one milk sample were A1 group members (intestinal). The results are pointed outHowever, according to our data, most of the isolates are part of the phylogenetic B2 and D (Extra-intestinal) groups. Because the B2 and D groupings are very common, there could be a potential risk of exposure to pathogenic E. coli., In this investigation, E. coli isolated was divided into phylogenetic groups according to the existence or absence of three genes: chuA , yjaA , and a DNA fragment termed TspE4.C2 .,The B2 3 group 3, in instance, was where a significant proportion of the detected isolates, stated that the presence of the B2 3 subgroup in farces, milk, and utensils could be an indication of contamination