Biology - Barcoding

We surveyed bacteria and fungi collected from a single household in Vancouver, Canada. Bacterial and fungal samples were obtained by culturing swabs from household surfaces and from tissue excised from mushroom fruiting bodies. The samples were amplified at the 16S rRNA gene (bacteria) or the ITS region (fungi), and sequenced by Sanger or Nanopore sequencing. Checking Basic Local Alignment Search Tool for Nucleotides (BLASTN; Altschul et al. 1990) against the nucleotide collection of National Center for Biotechnology Information (NCBI; Sayers et al. 2026) identified 27 of those 40 reads to genus or species level with reasonable confidence. All 27 sequences are now deposited in GenBank with confirmed accessions. The remaining 13 reads either returned no significant hit, matched only an uninformative "uncultured" clone, or fell below our confidence thresholds. The dataset is intended as a small, geographically precise addition to the growing record of household microbial and fungal diversity.