Gaoyuan-0423/pcr-workbench: WispLab paper anchor 2026-10-17 (qPCR module)

qPCR analysis module: ΔΔCt (Livak) and Pfaffl efficiency-corrected fold change, multi-reference-gene pooling, NTC/melting QC, group statistics (Welch t, one-way ANOVA + Dunnett).

Verification: independent reference implementation on real QuantStudio export — ΔΔCt, Livak and Pfaffl fold change agree within 1e-09, Welch t and ANOVA p-values within 1e-08, moderate-effect Dunnett within 2%; deep-tail Dunnett regression test included (real-data regression added after the tail-quadrature fix).