Published October 2, 2026 | Version v1

Data sets and R code associated with the study "Orsay virus infection is associated with reduced dispersal and alters cue-dependent food choice in Caenorhabditis elegans"

Description

1. Five independent experiments (BI, SC, AGR24, AGR48, PP) of food choice tests. Infected or non-infected animals placed at same distance from clean or OrV-contaminated food.  Number of worms that move towards each food source, or move randomly counted at different times. Experiments done in ERT54 wildtype or eol-1 mutant animals.
1.1. Dataset "Choice test 2 and 24 hpi.BI.xlsx":
Corresponds to experimenter BI.
In this experiment, non-contaminated food was prepared mixing bacterial media with an extract from non-infected animals to control for the effect of possible metabolites present in the OrV-contaminated food.
Column "Strain" with values "eol-1" y "ERT54". ERT54 are wild-type C. elegans.  "eol-1" are mutants for the eol-1 gene involved in olfactory learning.
Column "Treatment" with values 0 = non-infected controls and 1 = OrV-infected animals.
Column "Age" with values L3 = larval stage L3 and YA = young adults.
Column "Replicate" label to identify biological replicates (worm plates) on each of the "Strain" x "Treatment" x "Age" combinations.
Column "Choice" with values -1 = worms prefer clean food, 0 = worms show no preference and 1 = worms prefer OrV contaminated food.
Column "Time" exposition time 2 and 24 h. 
Column "Numworms" number of observed worms.

1.2. Dataset "Choice test 2 and 24 hpi.SC.xlsx":
Corresponds to experimenter SC.
Non-contaminated food was simply bacterial media.
Column "Strain" with values "eol-1" y "ERT54". ERT54 are wild-type C. elegans.  "eol-1" are mutants for the eol-1 gene involved in olfactory learning.
Column "Replicate" abel to identify biological replicates (worm plates) on each of the "Strain" x "Time" combinations.
Column "Choice" with values -1 = worms prefer clean food, 0 = worms show no preference and 1 = worms prefer OrV contaminated food.
Column "Time" exposition time 2 and 24 h. 
Column "Numworms" number of observed worms.

1.3. Dataset "Choice test 24 hpi.AGR y PP.xlsx":
Corresponds to experimenters AGR and PP with samples taken after 24 h by both.
This experiment was done only with ERT54 animals.
Non-contaminated food was simply bacterial media.
Column "Infected" with values 0 = non-infected controls and 1 = OrV-infected animals.
Column "Plaque" label to identify biological replicates (worm plates).
Column "Behavior" with values -1 = worms prefer clean food, 0 = worms show no preference and 1 = worms prefer OrV contaminated food.
Column "Numworms" number of observed worms.
Column "Block" 1 = Experimenter AGR and 2 = Experimenter PP.

1.4. Dataset "Choice test 48 hpi.AGR.xlsx":
Corresponds to experimenter AGR with samples after 48 h.
This experiment was done only with ERT54 animals.
Non-contaminated food was simply bacterial media.
Column "Infection" with values 0 = non-infected controls and 1 = OrV-infected animals.
Column "Plaque" label to identify biological replicates (worm plates).
Column "Behavior" with values -1 = worms prefer clean food, 0 = worms show no preference and 1 = worms prefer OrV contaminated food.
Column "Numworms" number of observed worms.

2. Four independent experiments (SC, PP, AGR, BI) measuring distance traveled by infected and non-infected animals.
2.1. Dataset "Distance 24 and 48 hpi.SC.xlsx"
Corresponds to experimenter SC.
Column "strain" with values "eol-1" y "ERT54". ERT54 are wild-type C. elegans.  "eol-1" are mutants for the eol-1 gene involved in olfactory learning.
Column "infection" with values 0 = non-infected controls and 1 = OrV-infected animals.
Column "hpi" exposition time 24 and 48 h. 
Column "distance" travelled distance (arbitrary units).

2.2. Dataset "Distance 24 hpi.PP.xlsx":
Corresponds to experimenter PP.
This experiment was done only with ERT54 animals with samples after 24 h.
Column "Infection" with values 0 = non-infected controls and 1 = OrV-infected animals.
Column "Plaque" label to identify biological replicates (worm plates).
Column "Distance" travelled distance (arbitrary units).

2.3. Dataset "Distance 65 hpi.BI.xlsx":
Corresponds to experimenter BI with samples after 65 h.
Column "strain" with values "eol-1" y "ERT54". ERT54 are wild-type C. elegans.  "eol-1" are mutants for the eol-1 gene involved in olfactory learning.
Column "age" with values L3 = larval stage L3 and YA = young adults.
Column "condition" with values control = non-infected worms and OrV = OrV-infected worms.
Column "replicate" label to identify biological replicates (worm plates).
Column "distance" travelled distance (arbitrary units).

2.4. Dataset "Distance 48 hpi.AGR.xlsx":
Corresponds to experimenter AGR with samples after 48 h.
Column "Infection" with values 0 = non-infected controls and 1 = OrV-infected animals.
Column "Plaque" label to identify biological replicates (worm plates).
Column "distance" measured as six concentric circumference areas equidistant from the plate center (1, 2, 3, 4, 5, 6).
Column "Numworms" number of observed worms on each area.

 

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Additional details

Funding

Agencia Estatal de Investigación
PID2025-169610NB-I00
Generalitat Valenciana
CIPROM/2022/59
Agencia Estatal de Investigación
FPU21/00410
Agencia Estatal de Investigación
PREP2022-000699
European Commission
MSCA 2024-PF-01-101207897

Dates

Created
2026-10-02
Data uploaded to Zenodo

Software

Development Status
Unsupported