Syngnathus spp. photos

This repository holds all of the photographs taken of Syngnathus floridae, S. fuscus, and S. scovelli prior to entering various mesocosm experiments. Morphometrics from the S. floridae and S. scovelli photographs have been previously published/analyzed at https://doi.org/10.1111/mec.17550 and https://doi.org/10.1086/671233 respectively. These photographs were re-analyzed using ImageJ alongisde the S. fuscus photos and the corresponding morphometrics were used in the manuscript "Comparing mating systems and the strength of sexual selection across three congeneric species of pipefish spanning the continuum of sexual dimorphism" (DOI: tbd).

Photographs of S. floridae and S. fuscus were taken in Sep-Dec 2020 and June-Aug 2021 respectively by NM Tosto. Photgraphs of S. scovelli were taken in Jul-Oct 2010 by E Rose.

There are three folders, one corresponding to each species:

1. syngnathus_floridae_meso_photos.zip - In this zipped folder there will be individual folders for each trial named Trial 1, Trial 2, etc. The individual trial folders then contain photographs of all male and female Syngnathus floridae that were included in that trial, taken right before entering. They are named in the following format:
   • Trial Number_Sex FishID_Type of photo, where trial number will look like TX, sex is either M or F and the FishID ranges from 1 - 8. For example a photo starting with T3_F1 would be female 1 from trial 3 and T3_M1 woulf me male 1 from trial 3. The final identifier indicates the type of photo taken:
     •  For females that will be indiciated with an "L" for the full length photo (allows you to measure from snout to caudal fin), "M" and "MB" for photos taken under black light to highlight the VIE tags used for identification, or "T" for a closer shot of the torso from snout to urogenital opening (helpful for more precise measurements of snout-vent length). For example, the T3_F1 fish would have the following photos: T3_F1_L, T3_F1_M, T3_F1_MB, T3_F1_T
     • For males, they have the same four listed above for females, however, we have an additional identifier of "B" for photos taken with the male's brood pouch gently pushed againt the glass (which allows us to measure things such as brood pouch area and length). 
   • All photos have a ruler included in the shot, aside from the "MB" photos which are a close-up of the VIE tag, which allows the user to set the scale for measuring.
2. syngnathus_fuscus_meso_photos.zip - In this zipped folder there will be individual folders for each trial named Trial 1, Trial 2, etc. The individual trial folders then contain photographs of all male and female Syngnathus fuscus that were included in that trial, taken right before entering. They are named in the same format as outlined above for S. floridae. However, note that as only 6 males and 6 females were used in these mesocosms (instead of the 8 as was done for S. floridae) the FishID will range from 1 - 6. 
   • Again, all photos have a ruler showing in the to allow the user to set the scale for measuring.
3. syngnathus_scovelli_meso_photos.zip - In this zipped folder there will be two separate folders for Male pipefih and Female (named "Males" and "Females). Whithin each of those folders will be individual folders for each trial named Trial1 - Trial8. The trial folders contain all of the Syngnathus scovelli male or female pipefish that were included in the trial, taken right before entering. They are names in the following format:
   • Females: C Trial Number_F FishID, where trial number and fish ID range from 1 - 8. There is one photo per female. For example the photo C2_F6 is female 6 from trial 2.
   • Males: C Trial Number_M FishID_Type of photo, where trial number and fish ID range from 1 - 8. There will be two photos for each male, one which ends in _L and is a full length photo (used to measure SVL, standard length, and torso depth) and one which ends in _B which was taken whith the male's brood pouch gently pushed against the glass (to get measurments such as brood pouch area and length). For example, the photos C4_M3_L and C4_M3_B are the two photos for male 3 in trial 4.
   • Again, all photos have a ruler showing in the picture to all the user to set the scale for measuring.