Published September 18, 2026
| Version v2
Improving eDNA detection in sponge natural samplers using blocking primers
Description
OTU Table Metadata
| File | OTU_Table.csv |
| Creator | Paul Dreger |
| Date | Apr 25 |
| Sequencer | MinION Mk1B, R10.4.1. flow cell technology - Oxford Nanopore Technologies |
| Software | decona v1.4, R 4.4.2 |
| Variables | Barcode: Sample identifier used for tracking samples during metabarcoding |
| uniqueID: Unique sequence ID using Barcode and SeqID | |
| SeqID: Identifier of the query sequence used in the BLAST search | |
| SseqID: Subject sequence identifier returned by BLAST | |
| Total_readcount: Total number of reads associated with the sample/barcode | |
| PercIdent: Percentage of identical bases between query and subject sequences | |
| Alignmentlength: Length of the alignment between the query and subject sequences | |
| Mismatch: Number of mismatched bases in the alignment | |
| Gapopen: Number of gap openings in the alignment | |
| QueryStart: Starting position of the alignment in the query sequence | |
| QueryEnd: Ending position of the alignment in the query sequence | |
| SubjectStart: Starting position of the alignment in the reference sequence | |
| SubjectEnd: Ending position of the alignment in the reference sequence | |
| Evalue: Expectation value; indicates the number of hits expected by chance | |
| Bitscore: Score indicating the quality/significance of the alignment | |
| Querylength: Total length of the query sequence | |
| Subjectlength: Total length of the reference sequence | |
| OTU: Operational Taxonomic Unit assigned based on sequence similarity | |
| TaxID: Taxon ID from BOLD/NCBI database | |
| Read_Count: Number of reads associated with the OUT | |
| domain: Domain name of highest ranking BLAST hit | |
| phylum: Phylum name of highest ranking BLAST hit | |
| class: Class name of highest ranking BLAST hit | |
| order: Order name of highest ranking BLAST hit | |
| family: Family name of highest ranking BLAST hit | |
| genus: Genus name of highest ranking BLAST hit | |
| species: Species name of highest ranking BLAST hit | |
| warning: Indicates whether sequence has been assigned to do different taxa with the same likelihood | |
| Sequence: Full consensus sequence after reclustering | |
| Primer: Primer used to inhibit host DNA amplification (G08R/G3F/None) | |
| SampleID: Unique sample ID | |
| Island: Island on which site is located | |
| Site: Location name | |
| Sample_or_Control: Label if sample is a true sample or control | |
| quant_reading: DNA quantity of PCR product used in sequencing (ng/µl) | |
| Abstract | This file is the OTU table generated by decona v1.4 from raw FASTQ files (for full command and parameters, see manuscript) containing each consensus sequence with its highest scoring BLAST hit based on E-Value, Percentage Identity and Alignment length. Taxonomic assignment was done using an adjusted version of the "Taxonomy assign after decona.R" script provided on https://github.com/karlijn-doorenspleet/decona-postprocessing. Raw FASTQ files as well as scripts used for assignment and downstream analyses will be made available in online nucleotide databases (which is to be determined) and GitHub, respectively. |
| Key words | metabarcoding, otus, blast, taxonomy |
| File type | CSV, UTF-8 encoding |
| License | CC BY-NC |
| Publication | This study |
|
sequencing_metadata.xlsx: Metadata file to assign fastq files to the samples they were originated from.
tax_table_contamination.csv: list of taxa found in the negative controls and removed from the other samples, when present. |