UV-Spectroscopy analytical method development and Validation of Teneligliptin Hydrobromide Hydrate

A simple, selective, and rapid UV-Vis spectrophotometer (UV-1800AD) method for the analysis of teneligliptin hydrobromide hydrate in bulk and in tablet dosage form has been developed and validated. The diluent of water: MeOH in the ratio of 80:20 ml v/v was delivered. Studies were performed on a UV-Vis Spectroscopy ℷmax at 245nm. The method is specific to teneligliptin hydrobromide hydrate and is able to quantify it from the tablet. The calibration curve was linear over the concentration range of 10-60μg/ml (r=0.999). The results of the analysis of method precision and intermediate precision were found within the acceptance criteria of +2. The proposed method is applicable to the routine analysis of Teneligliptin Hydrobromide Hydrate in bulk and in tablet dosage form.