Canonical Seurat objects for the bovine oviduct single-cell RNA-seq dataset

Reduced historical Seurat objects and processed gene-by-cell count matrices from the single-cell RNA-seq analysis of the bovine oviduct, comprising the infundibulum (INF), ampulla (AMP), and isthmus (IST).

The dataset contains six reduced historical Seurat objects: three corresponding to the all-cell analysis (INF, AMP, and IST) and three corresponding to the epithelial-cell analysis (INF, AMP, and IST). These objects serve as canonical checkpoints for reproducing the cluster structure, manual cell annotations, differential-expression analyses, and visualization outputs associated with the study. They retain the expression data, metadata, cluster identities, and UMAP embeddings required for downstream reproduction while omitting large intermediate components not required for the manuscript analyses.

The dataset additionally contains the gene-by-cell count matrices generated with PIPseeker v3.3 for the 15 biological samples. These matrices constitute the starting input for the computational re-analysis provided in the associated GitHub repository.

Analysis code, software environment information, and detailed reproduction instructions are provided in the associated GitHub repository.