Conserved candidate extracellular electron transfer machinery is widespread and transcriptionally active across deep peat profiles

This repository contains files that accompany the Fiorito et al. (2026) bioRxiv preprint.

These files include:

• Phylogenetic tree of 1081 MAGs of the study (MAGs_phylogenetic_tree.tre) and the underlying protein sequences (MAGs_Phylogenetic_tree_Aligned.faa);
• Phylogenetic tree of porins (All_porin_EET_NW_Tree.txt) and the underlying protein sequences (All_porin_50p_aligned.faa);
• Separate phylogenetic trees of the MHC families associated with candidate EET systems (EET_MHCs_trees.zip), together with the corresponding underlying aligned protein sequences (EET_MHCs_trees_proteins.zip);
• Phylogenetic tree comparing electroactive reference microorganisms with MAGs carrying candidate EET systems (EET_references_comparison_tree.tre) and the underlying protein sequences (EET_references_comparison_Aligned.faa);
• Phylogenetic tree of McrA proteins identified in MAGs and metagenomic contigs (McrA.treefile) together with the underlying aligned protein sequences (McrA_tree_aligned.faa);
• Genbank files of the 156 Acidobacteriota (Acidobacteriota_MHCs_extracellular_contigs_genbank.zip) and 54 Verrucomicrobiota (Verrucomicrobiota_MHCs_extracellular_contigs_genbank.zip) contigs carrying extracellular MHCs;
• AlphaFold3 predicted structural models of the proteins composing the 4 EET machinery systems (PDB_EET_components.zip).