Annotation file (gff3) from the killifish, Fundulus rathbuni (gill epithelium)

Annotation, gff3 file of the reference de novo transcriptome assembly from the killifish, Fundulus rathbuni created with the dammit pipeline using the Pfam, Rfam, OrthoDB databases in addition to Fundulus heteroclitus amino acids (RefSeq).

Fish were acclimated to either brackish or fresh water then exposed to an acute brackish water challenge. Transcriptome data from gill epithelium tissue were collected. A reference transcriptome assembly was generated from all individuals then used to analyze transcriptional responses to salinity.