Published August 22, 2026
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SOP13_Culture-Independent methods for microbiological analysis
Authors/Creators
- 1. H&TRC - Health and Technology Research Center - Escola Superior de Tecnologia da Saúde de Lisboa - IPL: Lisboa, Lisboa, PT
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2.
University of Essex
- 3. University of Essex Department of Biological Sciences
- 4. The University of Essex
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5.
Austrian Institute of Technology
- 6. Austrian Institute of Technology Center for Health and Bioresources
Description
This Standard Operating Procedure (SOP) covers the isolation and purification of genetic material (hereafter referred to as DNA extraction) from electrostatic dust collector (EDC) extracts and liquid impingement samples for subsequent culture-independent (molecular genetic) analysis. After DNA extraction, and depending on the agreements made with the project team and the analytical laboratories assigned to the sample analysis, the following methods will be used for further analysis:
1) Preparation of DNA amplicon libraries for high-throughput DNA sequencing
2) Quantification of taxonomic gene markers with qPCR