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Published August 11, 2026 | Version v1.0.0

MetaBarFlow: a generalised, marker-agnostic metabarcoding pipeline

Authors/Creators

Description

MetaBarFlow is a reproducible Illumina paired-end amplicon metabarcoding pipeline (merge, quality check, primer trimming, DADA2 ASV inference, and taxonomy assignment) that is configurable for any marker, with ready-to-use configurations for 18S (protists), ITS (fungi), and 16S (bacteria/archaea). It also includes a downstream statistics stage (filtering, rarefaction, and dataset-quality diagnostics).

Notes

If you use MetaBarFlow, please cite it using the metadata below.

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DavidSinger85/MetaBarFlow-v1.0.0.zip

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