Published July 24, 2026 | Version v1

First eDNA-based record of Carcharhinus melanopterus (Quoy & Gaimard, 1824) (Carcharhiniformes, Carcharhinidae) from Mantanani Island, Sabah, Malaysia

  • 1. Institut Marin Borneo, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
  • 2. JSK Mantanani Island Resorts, Pulau Mantanani Besar, Kota Belud, Malaysia
  • 3. Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, Japan

Description

The first molecular occurrence record of the Blacktip Reef Shark, Carcharhinus melanopterus (Quoy & Gaimard, 1824), is documented from Mantanani Island, Sabah, Malaysia, based on environmental DNA (eDNA) metabarcoding. Surface seawater collected from two seagrass sites on 9 May 2024 and analyzed using MiFish 12S rRNA primers. A single 155-bp operational taxonomic unit matching C. melanopterus with 100% identity to MitoFish reference sequences was recovered exclusively from one of the sampled sites (20,881 reads). No reads were detected at the other site or in negative controls. Sequence data are deposited in NCBI SRA (BioProject PRJNA1460790).

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