ASAP CRN Cloud Team
Published July 23, 2026 | Version v0.1
Dataset Open

Single-nucleus RNA sequencing of miBrains, a human brain tissue model

Authors/Creators

  • 1. Icahn School of Medicine, Mount Sinai, New York, NY, USA; Department of Stem Cell Biology and Regenerative Medicine, Mount Sinai, New York, NY, USA; Nash Family Department of Neuroscience, Mount Sinai, New York, NY, USA; Friedman Brain Institute, Mount Sinai, New York, NY, USA; Ronald M. Loeb Center for Alzheimer's Disease, Mount Sinai, New York, NY USA; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
  • 2. Icahn School of Medicine, Mount Sinai, New York, NY, USA; Department of Stem Cell Biology and Regenerative Medicine, Mount Sinai, New York, NY, USA; Nash Family Department of Neuroscience, Mount Sinai, New York, NY, USA; Friedman Brain Institute, Mount Sinai, New York, NY, USA; Ronald M. Loeb Center for Alzheimer's Disease, Mount Sinai, New York, NY USA
  • 3. Icahn School of Medicine, Mount Sinai, New York, NY, USA; Friedman Brain Institute, Mount Sinai, New York, NY, USA; Department of Psychiatry, Mount Sinai, New York, NY, USA; Department of Genetics and Genomic Sciences, Mount Sinai, New York, NY, USA; Center for Disease Neurogenomics, Mount Sinai, New York, NY, USA.

Description

This dataset contains snRNA-seq data generated from human induced pluripotent stem cell-derived 3D brain tissue. Specifically, this is a multicellular integrated human brain tissue (miBrain) containing multiple brain cell types. The dataset includes three experimental conditions: control miBrains, miBrains with overexpression and mutation of the SNCA gene (A53T) and the APOE3/3 genotype, and miBrains with SNCA-A53T and APOE4/4 genotype. The SNCA manipulation is present only in the neurons, whereas the APOE genotype manipulation is identical in all cell types within the same sample. Control miBrains express endogenous SNCA and harbor APOE3/3. All miBrains are isogenic.

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vangheluwe-ipsc-sn-rnaseq-3dorga-apoe-asyn_README.pdf

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Additional details

References