Published July 21, 2026 | Version v1

Isolation and Characterization of soil bacteria from Kaunia and Jamun series of Bangladesh

  • 1. Soil Resource Development Institute, Ministry of Agriculture, Bangladesh
  • 2. Department of Agricultural Extension, Ministry of Agriculture, Bangladesh

Description

ABSTRACT:

Soil bacteria are essential for maintaining soil fertility and ecosystem functioning; however, information on the culturable bacterial diversity of many Bangladeshi soil series remains limited. This study aimed to isolate, enumerate, and characterize culturable bacteria from the Kaunia and Jamun soil series of Bangladesh. Soil samples were collected from agricultural fields in Mithapukur, Rangpur, and bacterial isolates were obtained using serial dilution and spread plate techniques. Bacterial populations were quantified as colony-forming units (CFU), while colony morphology and staining characteristics were used for phenotypic characterization. Kaunia soil contained a slightly higher bacterial population (4.6 × 10⁸ CFU g⁻¹ soil) than Jamun soil (4.4 × 10⁸ CFU g⁻¹ soil). Nine and seven distinct bacterial colonies were isolated from Kaunia and Jamun soils, respectively. The isolates showed considerable variation in colony morphology and included both Gram-positive and Gram-negative bacteria, with several exhibiting spore- and capsule-forming abilities. These findings provide baseline information on the culturable bacterial diversity of the Kaunia and Jamun soil series and support future molecular and functional studies for sustainable agricultural applications.

Keywords: Soil bacteria; Kaunia soil series; Jamun soil series; Colony, morphology; Bacterial characterization.

Files

MSIJSAFS392026 GS.pdf

Files (658.3 kB)

Name Size Download all
md5:40b0a6a726785f8d59b37bfb11dd0151
658.3 kB Preview Download

Additional details

References

  • Stevenson FJ. Cycles of soil. New York: John Wiley & Sons; 1986.
  • Brady NC, WeilRR. The nature and properties of soils. 13thed. London: Pearson Education Ltd.; 2002. p. 156-198.
  • Joklik WK, Willett HP, Amos DB, Wilfert CM. Zinsser microbiology. 20th ed. Norwalk (CA): Appleton & Lange;1992.
  • Alexander M. Introduction to soil microbiology. 2nd ed. New York: John Wiley & Sons; 1997.
  • Ruangpan L, Tendencia EA. Laboratory manual of standardized methods for antimicrobial sensitivity tests forbacteria isolated from aquatic animals and environment. Tigbauan, Iloilo, Philippines: Southeast Asian Fisheries Development Center, Aquaculture Department; 2004. p. 3–11. http://hdl.handle.net/10862/1616
  • Salle AJ. Fundamental principles of bacteriology. 7th ed. NewDelhi: Tata McGrawHill Publishing Co. Ltd.; 1973. https://www.cabidigitallibrary.org/doi/full/10.5555/19781939164
  • Mishra RR. Soil microbiology. 1st ed. New Delhi: CBS Publishers & Distributors; 2000.
  • Chowdhury MH. Isolation and characterization of bacteria of Amjhupi and Ishurdi soil series of Bangladesh. Int J Appl Chem Biol Sci. 2020;1(4):33-39.
  • Chowdhury MH. Effect of heavy metal on bacterial population and diversity of Gopalpur soil series. Int J Appl Chem Biol Sci. 2020;1(4):20-24.
  • Marzan LW, Hossain M, Mina SA, Akter Y, Chowdhury AMA. Isolation and biochemical characterization of heavy metal– resistant bacteria from tannery effluent in Chittagong city, Bangladesh: bioremediation viewpoint. Egypt J Aquat Res. 2017;43(1):65-74. https://doi.org/10.1016/j.ejar.2016.11.002
  • Salam MA, Hossain MS, Ali ME, Asad MA, Ali MH. Isolation and characterization of arsenic-resistant bacteria from different environments in the south-west region of Bangladesh. Res J Environ Sci. 2009;3(1):110-115. https://scialert.net/fulltext/fulltextpdf.php? pdf=academicjournals/rjes/2009/110- 115.pdf
  • Chowdhury MH. Bacterial population and characterization of Sonatala and Bajoa soil series of Bangladesh. Int J Appl Chem Biol Sci. 2022;3(2):44-49. https://identifier.visnav.in/1.0001/ijacbs-22c-07007/
  • Kabir MR, Hasan SMK, Chy MAH, Miah MS, Billah SM, Chowdhury MH. Assessment of soil bacterial population and characterization of Akdala and Nachol series of Bangladesh. GRS J Multidiscip Res Stud. 2025;2(7):1-5. https://msipublishers.com/wpcontent/uploads/2026/04/MSIJMR4542026-GS.pdf
  • Chowdhury R, Dhar K, Ahamed F, Alam S, Uddin M. Biodegradation of anthracene and phenanthrene by bacteria isolated from oil-contaminated soil of Bangladesh. Chem Ecol. 2017;33(9):843-855.https://doi.org/10.1080/02757540.2017.1382481
  • Mahmud MK, Azad MAK, Awal A, Tina SA, JD, Salwa M, et al. Isolation and characterization of bacteria of Amnura and Baliadangi soil series of Bangladesh. Int J Appl Chem Biol Sci. 2025;6(1):1-6. https://identifier.visnav.in/1.0001/ijacbs-24l25002/
  • Hossain MA, Ahmed S, Hoque S. Abundance and distribution of Bacillus thuringiensis in the agricultural soil of Bangladesh. J Invertebr Pathol. 1997;70(3):221-225. https://doi.org/10.1006/jipa.1997.4694
  • Khan M, Rashid H. Enumeration, isolation and identification of nitrogen-fixing bacterial strains at seedling stage in rhizosphere of rice grown in non-calcareous grey flood plain soil of Bangladesh. J Fac Environ Sci and Tech. 2008;13(1):97- Page 17 of 18 DOI: 10.5281/zenodo.21467739 101. https://ousar.lib.okayamau.ac.jp/files/public/1/12834/20160527192202548508/13_0 97_101.pdf
  • Bhuiya ZH, Walker N. Autotrophic nitrifying bacteria in acid tea soils from Bangladesh and Sri Lanka. J Appl Bacteriol. 1977;42(2):253-257. https://doi.org/10.1111/j.1365-2672.1977.tb00690.x
  • Dubey RC, Maheshwari DK. A text book of microbiology. New Delhi: S. Chand & Co. Ltd.; 1999.
  • Prescott LM, Harley JP. Laboratory exercises in microbiology. 5th ed. New York: McGraw-Hill; 2002
  • Cappuccino JG, Sherman N. Microbiology: a laboratory manual. 4th ed. Menlo Park (CA): Benjamin/Cummings Publishing; 1999.
  • Shaha SC. Microbiology. 1st ed. Dhaka: Kobir Publications; 2003.
  • Chowdhury MH, Kibria KQ, Tisha SS, Islam MS. Isolation andcharacterization of bacteria of Sara and Mirpur soil series of Bangladesh. Khulna Univ Stud. 2013;11(1-2),12(1-2):36-41. https://doi.org/10.53808/KUS.2013.11and12.1214-L
  • Fakruddin MD, Sarker N, Ahmed MM, Noor R. Protein profiling of Bacillus thuringiensis isolated from agro-forest soil in Bangladesh. J Mol Biol Biotechnol. 2012;20:139-145.
  • Ahsan MM, Khan MFR, Rahman MB, Chowdhury SMZH, Parvej MS, Jahan M, et al. Investigation into Bacillus anthracisspore in soil and analysis of environmental parameters related to repeated anthrax outbreak in Sirajganj, Bangladesh. Thai J Vet Med. 2013;43(3):449-454. https://doi.org/10.56808/2985-1130.2505
  • Shishir A, Roy A, Islam N, Rahman A, Khan SN, Hoq M. Abundance and diversity of Bacillus thuringiensis in Bangladesh and their cry genes profile. Front Environ Sci. 2014;2:20. https://doi.org/10.3389/fenvs.2014.00020
  • Shishir A, Akter A, Hassan MH,Kibria G, Ilias M, Khan SN, et al. Characterization of locally isolated Bacillus thuringiensisfor the development of eco-friendly biopesticides in Bangladesh. J Biopesticides. 2012;5:216. https://jbiopestic.com/journals/Vol_5_0_216_222F.pdf
  • Fasanella A. Bacillus anthracis, virulence factors, PCR and interpretation of results. Virulence. 2015;4(8):659-660. https://doi.org/10.4161/viru.26517
  • Rume FI, Affuso A, Serrecchia L, Rondinone V, Manzulli V, Campese E, et al. Genotype analysis of Bacillus anthracisstrains circulating Bangladesh. PLoS One. 2016;11(4): e0153548. https://doi.org/10.1371/journal.pone.0153548