Published December 21, 2025 | Version v1

Exhausted culture media reuse in autotrophic microalgae production: Optimization and modelling of Pseudococcomyxa simplex cultures

Description

This publication investigates the reuse of exhausted culture medium as a strategy to reduce water consumption in autotrophic microalgae production. Semi-continuous cultivations of Pseudococcomyxa simplex were performed under different medium recycling conditions, and the experimental results were integrated with mathematical modelling and process simulation to optimize purge ratio and nutrient management. The study demonstrates that culture medium recycling can substantially decrease the water footprint while maintaining stable biomass productivity, providing practical guidelines for the design of more sustainable and resource-efficient microalgal cultivation processes.

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