Wound Healing Assay Time-Lapse Series

Qualified researchers may request access for validation purposes by contacting the corresponding author via institutional email. Data sharing requires a formal Data Transfer Agreement (DTA).

Dataset Metadata: Wound Healing Assay Time-Lapse Series

1. General Identification

• Dataset Title: Time-Lapse Microscopy Dataset for Cellular Migration and Wound Healing Analysis
• Version: 1.0.0
• Data Content: In-vitro scratch assay images capturing cell migration over a 72-hour period.
• Keywords: Cell Migration, Wound Healing Assay, Scratch Assay, Time-lapse Microscopy, Computer Vision.

2. Access and Rights (Controlled Access)

• Access Status: Controlled Access
• Reason for Restriction: Data contains sensitive experimental parameters and is part of an ongoing institutional research project.
• Metadata DOI/URL: https://zenodo.org/records/19653335
• Access Request Procedure: Qualified researchers may request access for validation purposes by contacting the corresponding author via institutional email. Data sharing requires a formal Data Transfer Agreement (DTA).

3. Technical Metadata (Dataset Structure)

4. Data Dictionary (Naming Convention)

The file naming system is as follows: {Time_Point} ({Replicate_ID}).JPG

• Example: 24 (10).JPG -> Image captured at the 24th hour for replicate sample number 10.

5. Experimental Context

• Imaging Platform: Olympus CX31Light Microscope / Olympus E330 Digital Camera
• Magnification: x20
• Cell Line: Human Breast Adenocarcinoma - MCF-7 / Human Hepatocellular Carcinoma - HepG2

6. Summary Statistics for Reviewers

• Dataset Integrity: The dataset includes complete sequences for all time points (0-72h).
• Target-to-Background: High-contrast boundaries for optimal segmentation performance.