ANALYTICAL MEHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF CILNIDIPINE AND METOPROLOL BY UHPLC IN SOLID DOSAGE FORMS

Abstract
Literature review reveals that there is no analytical method reported for the analysis of Cilnidipine and
Metoprolol by UHPLC in solid dosage form. Spectrophotometer, HPLC and RP-HPLC are the reported
analytical methods for compounds either individually or in combination with other dosage form.
Hence, it was felt that, there is a need of new analytical method development for the simultaneous
estimation of Cilnidipine and Metoprolol by UHPLC in pharmaceutical dosage form. Present work is
aimed to develop a new, simple, fast, rapid, accurate, efficient and reproducible Analytical mehod
development and validation for the simultaneous estimation of cilnidipine and metoprolol by UHPLC
in solid dosage forms. The developed method will be validated according to ICH guidelines. A simple,
Accurate, precise method was developed for the simultaneous estimation of the Cilnidipine and
Metoprolol in solid dosage form. Retention time of Cilnidipine and Metoprolol were found 0.61min
and 1.20 min respectively. System precision. %RSD of the Cilnidipine and Metoprolol were found
0.89 and 1.01 respectively. %Assay were obtained as 99.86% for Cilnidipine and 99.39 % for
Metoprolol. Regression equation of Cilnidipine is y = 4.4172x + 1.976. Regression equation of
Metoprolol is y=64.541x+ 79.588. Retention times were decreased and that run time was decreased,
so the method developed was simple and economical that can be adopted in regular Quality control
test in Industries.