Published June 10, 2026 | Version v1
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FORMULATION AND CHARACTERIZATION OF HERBAL-BASED ANTIDANDRUFF SHAMPOO AND ASSESSMENT OF ITS ANTIFUNGAL ACTIVITY

  • 1. Student, PG & Research Department of Microbiology, Dwaraka Doss Goverdhan Doss Vaishnav College, Chennai, Tamil Nadu, India.
  • 2. Quality Analyst – Microbiology Department, National Food Testing Laboratory (FSSAI & NCML PPP), Chennai, Tamil Nadu, India.
  • 3. Assistant Professor, PG & Research Department of Microbiology, Dwaraka Doss Goverdhan Doss Vaishnav College, Chennai, Tamil Nadu, India.

Description

Abstract

Dandruff is a widespread scalp issue affecting over 50% of the global population. The rising demand for natural and herbal products in the cosmetic industry has prompted the exploration of plant-based ingredients for hair care formulations. The purpose of this study is to formulate antidandruff shampoo using a combination of traditional herbs known for their therapeutic, antimicrobial, and scalp-nourishing properties. The extraction and incorporation of these herbal components were optimized to ensure the effectiveness of the shampoo in addressing dandruff-related concerns. The bioactive components from neem, fenugreek seed, aloe vera, and clove were extracted using different solvents. Additionally, the presence of active compounds in the herbal extracts was confirmed through phytochemical screenings. Fourier transform infrared spectroscopy (FTIR) detected the presence of bioactive chemicals and functional groups, such as nitro compounds and amide groups, in the plant extracts. Two different combinations of anti-dandruff shampoos were formulated. The formulated shampoo was characterized physically and chemically. The shampoo's antibacterial activity was tested against Staphylococcus aureus and Staphylococcus epidermidis and for antifungal activity, it was tested against Malassezia furfur, a fungus associated with dandruff using the agar well diffusion method. Parameters such as pH, foaming agents, solid content, and organoleptic properties were analyzed to ensure product stability and user acceptance. The findings of this study contribute to the development of herbal-based hair care products with a specific focus on addressing dandruff issues. The characterization results offer insights into the formulation's stability and quality, while the antifungal activity assessment provides valuable information on its potential therapeutic benefits. The herbal antidandruff shampoo presented in this study represents a promising natural alternative for individuals seeking effective and plant-derived solutions for scalp health.

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