Published June 5, 2026 | Version v0.1
Software Restricted

Lyso-pHrOG analysis pipeline

Authors/Creators

  • 1. Charité – Universitätsmedizin Berlin, Neurosciences Research Center; NeuroCure Cluster of Excellence; Institute of Biology, Experimental Biophysics, Humboldt-University zu Berlin
  • 2. NeuroCure Cluster of Excellence, Charité – Universitätsmedizin Berlin; Institute of Biology, Experimental Biophysics, Humboldt-University zu Berlin

Description

This repository contains the analysis pipeline used to generate Figures 4, 5, S6, S10, S11, S12, and S13 of the paper. The scripts process single-vesicle tracking data exported from TrackMate (two-channel fluorescence microscopy), converting intensity ratios to absolute pH values using a Hill function calibration anchored per track to an NH4Cl plateau. The pipeline quantifies light-evoked lysosomal acidification and alkalization kinetics, response amplitudes, and spatial distributions relative to the nucleus.

Notes

If you use this software, please cite both the software and the associated paper (see preferred-citation below).

Files

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Additional details

Related works

Is supplement to
Software: https://github.com/alejandrcastro/GernerWaltherEtAl2026/tree/v0.1 (URL)

Software

Repository URL
https://github.com/alejandrcastro/GernerWaltherEtAl2026