Antidiabetic, Anti Inflammatory, DNA Oxidative Modification And RBC Ghost Membrane Stability Of Caesalpinia Crista Seed Coats And Its Silver Nanoparticles

The current work reveals the manufacturing of silver nanoparticles (AgNps) mediated by caesalpinia crista seed coat extract and its impact on inflammation and diabetes caused by oxidative stress. Additionally, Aq CCSC AgNPs demonstrated a moderate level of anti-inflammatory properties when tested with PBMCS. Furthermore, the anti-diabetic properties of Aq CCSC AgNPs were demonstrated by their lack of action on α-amylase and α-glucosidase (20%) activity. To sum up, Aq CCSC AgNPs seem to be a promising option for controlling inflammation, diabetes, and oxidative stress.Although there are several methods for measuring oxidative DNA damage, the comet assay is among the most precise and sensitive due to its relative lack of artefacts.. The comet assay, also known as single cell gel electrophoresis, was developed as a sensitive technique for identifying breakage in DNA strands at the individual cell level. Additionally, it can be used to evaluate the antioxidant state of cells.The comet assay has evolved into a vital tool for genotoxicity assessment and human biomonitoring.An RBC (Red Blood Cell) ghost membrane stability test, which frequently uses hypotonic stress or mechanical force (shear), gauges how effectively a material prevents red blood cell membranes from rupturing (lysis) or changing in form. By measuring changes in membrane integrity, protein retention, or vesicle size, "ghosts" (hemoglobin-free membranes) are prepared and their resistance to disruption is tested. This process can be used as a model for anti-inflammatory effects, drug delivery system evaluation, or understanding cell mechanics.But ghost membrane and oxidative stress shows negative results against AqCCSC extract,calcined,uncalcined AgNps.