Cladosporium mambaiense J. F. S. A. Prazeres, P. H. Félix-Oliveira, E. O. Fonseca, Souza-Motta & J. D. P. Bezerra sp. nov.

Fig. 12

Etymology.

Named after Mambaí, the municipality where most of the caves studied here are located.

Typification.

BRAZIL • Goiás state, Mambaí municipality, Lapa do Córrego das Dores cave, 14°25'45"S, 46°13'10"W, isol. from air, May 2023, coll. J. F. S. A. Prazeres, E. O. Fonseca & J. D. P. Bezerra, isol. E. O. Fonseca [holotype URM 9104 HT (preserved in metabolically inactive state), UFG 39351 (microscopic slide ex-holotype), culture ex-type URM 9104 = FCCUFG 71].

Description.

Hyphae branched, septate, hyaline to light brown, smooth, thick-walled. Conidiophores solitary, semimacronematous to macronematous, branched, hyaline to light brown, arising terminally and laterally from hyphae, erect to flexuous, often filiform, non-nodular, regularly septate, septa often darkened and thick, 110–554 × 2.5–4 μm. Conidiogenous cells integrated, cylindrical-oblong, terminal and intercalary, with 1–4 darkened apical loci, 7–29 × 3–5 μm. Ramoconidia often formed, subcylindrical, cylindrical-oblong to oblong, aseptate, smooth, 16.5–25 × 2–5 μm. Secondary ramoconidia ellipsoid, fusiform to subcylindrical or cylindrical, aseptate, hyaline to light brown, smooth or minutely verrucose, with slightly thickened walls, 11–30 × 2–3.5 μm. Intercalary conidia in branched chains, branching in all directions, without regular septation, ovoid, ellipsoid, small fusiform, 7–13 × 2.5–4 μm; terminal conidia small, fusiform to ellipsoid, 3–5 × 1–3 μm.

Culture characteristics.

Colonies average diameters (mm) of 29, 44, 40, and 37 on SNA, PDA, MEA, and OA, respectively. On SNA, the colonies are greenish olivaceous, with a smooth, compact centre and a lighter, well-defined edge, reverse olivaceous black, smooth, compact aerial mycelium, without prominent exudates. On PDA, olivaceous buff, velvety texture, distinct radial groove pattern, reverse olivaceous black, smooth aerial mycelium, without prominent exudates. On MEA, olivaceous grey, with pronounced radial furrows and a slightly lighter outer ring, reverse olivaceous black, compact, smooth aerial mycelium, without prominent exudates. On OA, grey olivaceous, with a smooth texture, faint central ring and a subtle radial pattern, reverse olivaceous black, smooth aerial mycelium, without prominent exudates. Colonies average diameters (mm) of 44, 50, and 47 on CYA, DG 18, and MY 40 G, respectively. Colony growth on PDA was observed at 30 ° C, and no growth was observed at 36 ° C.

Additional material examined.

BRAZIL • Goiás state, Vila Propício municipality, Lapa do Boqueirão cave, isol. from air, May 2022, coll. P. H. Félix-Oliveira & J. D. P. Bezerra, isol. P. H. Félix-Oliveira (isolate FCCUFG 147).

Notes.

Cladosporium mambaiense sp. nov. (C. cladosporioides SC) is morphologically (Table 3) and phylogenetically (Figs 3, 9) related to C. angustisporum. Cladosporium mambaiense differs morphologically from C. angustisporum, a species described from Alloxylon wickhamii (Proteaceae) in Australia (Bensch et al. 2012), mainly by the size and shape of its reproductive structures. Cladosporium mambaiense has longer (110–554 × 2.5–4 μm) and erect conidiophores, whereas the conidiophores of C. angustisporum are smaller (22–280 × 2.5–4 μm). Ramoconidia of C. mambaiense are subcylindrical, smooth, and smaller (16.5–25 × 2–5 μm), whereas those of C. angustisporum are cylindrical and measure 18–55 × 2.5–3 μm. The closest phylogenetic species, C. angustisporum (CBS 125983), differs from C. mambaiense (URM 9104) by 12 bp in ACT, 14 bp in TEF 1 - α, and 15 bp in TUB; the ITS sequence shows no base pair difference. The RPB 2 sequence was not obtained from the ex-type strain of C. angustisporum (CBS 125983), but it was obtained from C. angustisporum (CPC 22345), also included in the analysis, and a 32 bp difference was observed. The PHI test did not indicate evidence of genetic recombination (Fig. 6 C). These isolates were found at sampling points inside the caves.