Flow cytometry data: CRISPR/Cas9 transcription-factor knockout validation in intratumoral CD8+ OT-I T cells (B16-OVA)

Raw flow cytometry (.fcs) files and analysis summaries for the flow-cytometry validation of transcription-factor (TF) knockouts (Crem, Batf, Tbx21, Zmiz1, and triple combinations) nominated by an in vivo CRISPR/Cas9 screen, associated with the manuscript 'Transcriptional Modulons as Blueprints for T Cell State Engineering'. OVA-specific OT-I CD4Cre Cas9-eGFP CD8+ T cells were transduced with retroviral sgRNA constructs, expanded in vitro, and adoptively transferred into B16-OVA tumor-bearing B6J.Cas9-EGFP mice. Tumor-infiltrating and splenic transferred T cells were analyzed by flow cytometry (BD LSRFortessa) to quantify progenitor-exhausted (Tpex) and terminal-exhausted (Tex) subsets. 44 .fcs files: 8 in vitro phenotyping, 35 tumor (7 conditions x 5 mice, day 3 post-ACT), 1 spleen (an instrument setup/test tube was excluded). Includes MIFlowCyt-structured metadata (FlowRepository_MIFlowCyt_metadata.md) and a per-file annotation table (FlowRepository_file_mapping.csv). Note: flow cytometry data are out of scope for NCBI GEO; FlowRepository was temporarily suspended for new submissions, so the data are archived here with MIFlowCyt annotation. Related CRISPR/Cas9 sgRNA-screen sequencing data are deposited in GEO (accession pending).