DEVELOPMENT AND VALIDATION OF SIMULTANEOUS EQUATION METHOD FOR ESTIMATION OF EMPAGLIFLOZIN AND SITAGLIPTIN PHOSPHATE MONOHYDRATE IN BULK AND DOSAGE FORM BY UV SPECTROSCOPY

ABSTRACT:
For measuring EMPA and SPM in bulk and pharmaceutical forms to created a accurate and affordable UV
spectrophotometric method. Both tested medications in a water and methanol mix (70:30). EMPA absorbs light at 224
nm and SPM at 267 nm, which we found by scanning light wavelengths from 200 to 400nm. The method shows results
for both EMPA and SPM across certain concentration ranges: 4–20 μg/mL for EMPA with a correlation coefficient of
0.99579 and 16–80 μg/mL for SPM with a correlation coefficient of 0.99295. And checked that our method meets ICH
requirements for Linearity, Accuracy, Precision, Robustness our method is precise with a percentage standard deviation
(%RSD) of less than 2% for all test parameters. This technique is suitable, for quality control analysis of mixed dose
forms containing EMPA and SPM.